FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464
Total Bases501.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC69820.150115473441108No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC62217.956120092378754No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG38110.998845265588916No Hit
GTTGGCGGATACCCTTCCTCTACGCCTAAGGTCACCGCAGTAAACAAAGG1875.3983833718244805No Hit
GTTGGCGGATACCCTTGTGAAAGCAAAGTCTGAATAAATAAGTAGCCTCT1193.435334872979215No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1113.2043879907621244No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1032.9734411085450345No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCAAACTGGT722.0785219399538106No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT391.1258660508083143No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG371.0681293302540416No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT150.4330254041570438No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG150.4330254041570438No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTAAGATGTGGC140.40415704387990764No Hit
GTTGGCGGATACCCTTCCTCTGCACAATGTCTGGGGTGGGAGTTGGGAAG140.40415704387990764No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA130.37528868360277134No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT130.37528868360277134No Hit
TACCTCAGTTTGCCCCCATGTTGTGTTCATGGGCACACAAGTCCCCAAAG120.3464203233256351No Hit
GTTGGCGATACCCTTCCTCTACGCCTAAGGTCACCGCAGTAAACAAAGGC110.3175519630484988No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.3175519630484988No Hit
GTTGGCGGATACCCTTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAAT110.3175519630484988No Hit
GTTGGCGGATACCCTTCCATGGTGAACAGCCTCTGGCATGTTGGAACAAT100.28868360277136257No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC90.25981524249422633No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA90.25981524249422633No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.23094688221709006No Hit
GTTGGCGGATACCCTTCCTTTTTTATCCTCCACTACAATCACAATCTCTG80.23094688221709006No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.23094688221709006No Hit
GTTGGGGATACCCTTCCTCTACGCCTAAGGTCACCGCAGTAAACAAAGGC70.20207852193995382No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA70.20207852193995382No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC60.17321016166281755Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGTCCTCAACTG60.17321016166281755No Hit
GTTGGCGGATACCCTTGGGAAGTAGTTTTTGGTACCCTTTGGAATAATGT60.17321016166281755No Hit
TACCTCAGTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAG60.17321016166281755No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT60.17321016166281755No Hit
GTTGGCGGATACCCTTGGACGTAGATGTGGCAAGATGGCAAACTGGTGTA50.14434180138568128No Hit
GTTGGCGGATACCCTTCCGATCGGCCGAGGGCAACGGAGGCCATCGCCCG50.14434180138568128No Hit
GTTGGCGGATACCCTTGATGGCATAAGATGTGGCAAGATGGCAAACTGGT50.14434180138568128No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT50.14434180138568128No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA50.14434180138568128No Hit
GTTGGCGGATACCCAGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG50.14434180138568128No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT50.14434180138568128No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC50.14434180138568128No Hit
GTTGGCGGATACCCTGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG40.11547344110854503No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGAAGATGGCAAACTGGTG40.11547344110854503No Hit
GTTGGCGGATACCCTTCCATCTTCACAAACAGATGTGGCAAGATGGCAAA40.11547344110854503No Hit
GTTGCGGATACCCTTCCTCTACGCCTAAGGTCACCGCAGTAAACAAAGGC40.11547344110854503No Hit
CGAAGTAGACCAGCGGCACCTCGGCCCCACCCTGGACCTTAGCTCAGCAC40.11547344110854503No Hit
TACCTCAGTTTGCCCCCATGGGGCTCAGCCTTCCCCTGAGTACTAGCGTC40.11547344110854503No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.11547344110854503No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACGATGTGGC40.11547344110854503No Hit
GTGTCGTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT40.11547344110854503No Hit
TACCTCAGTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCGTC40.11547344110854503No Hit
GTTGGCGGATACCAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG40.11547344110854503No Hit
GTTGGCGGATACCCTTAATCAAGGCTATTGGTACTGAACCAGATACAGAT40.11547344110854503No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT40.11547344110854503No Hit
GTTGGCGGATACCCTAGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT40.11547344110854503No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG40.11547344110854503No Hit
GTTGGCGGATACCTTCCTCTACGCCTAAGGTCACCGCAGTAAACAAAGGC40.11547344110854503No Hit
GTTGGCGGTACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTGG40.11547344110854503No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTACAGAGTTT40.11547344110854503Clontech SMART CDS Primer II A (96% over 26bp)
CGAAGTAGACCAGCGGCATCTCGGACCCACCCTGGACCTTAGCTCAGCAC40.11547344110854503No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.11547344110854503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)