FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5503
Total Bases797.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT392671.34290387061603No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC2324.21588224604761No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1693.0710521533708888No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1332.4168635289841904No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA971.7626749045974923No Hit
TGCCATCTTGCCACATCTTCAAAGTTGAAAAGAACTTTGAAGAGAGAGTT571.0357986552789387No Hit
TGCCATCTTGCCACATCTTGAGCGGAGGATGGAAGGGTATCCGCCAACTT220.39978193712520443No Hit
TGCCATCTTGCCACATCTTGAGCGGAGGACGGAAGGGTATCCGCCAACTT160.2907504997274214No Hit
TGCCATCTTGCCACATCTCGAGCGGAGGACGGAAGGGTATCCGCCAACTT150.27257859349445757No Hit
TGCCATCTTGCCACATCTCGAGCGGAGGATGGAAGGGTATCCGCCAACTT140.25440668726149374No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG140.25440668726149374No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC140.25440668726149374No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA110.19989096856260222No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT110.19989096856260222No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA110.19989096856260222No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT110.19989096856260222No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT100.18171906232963836No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT100.18171906232963836No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA100.18171906232963836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG100.18171906232963836No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG90.16354715609667453No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC90.16354715609667453No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT80.1453752498637107No Hit
TGCCATCTTGCCACATCTTGCAAGGGTATCCGCCAACTTGAGGAGACCAA80.1453752498637107No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT70.12720334363074687No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT70.12720334363074687No Hit
TGCCATCTTGCCACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGT70.12720334363074687No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT70.12720334363074687No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT60.10903143739778302No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC60.10903143739778302No Hit
TGCCATCTTGCCACATCTTTTATAAAGGGTATCCGCCAACTTGAGGAGAC60.10903143739778302No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT60.10903143739778302No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT60.10903143739778302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)