FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26543
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1685463.49696718532193No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC390714.71951173567419No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA10674.019892250310816No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT5231.9703876728327618No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA4221.5898730362054025No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3741.4090343970161625No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2981.1227065516331989No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1020.3842821082771352No Hit
TGCCATCTTGCCACATCTTACTAGGACGCAAAGGGGAAAGGGGGCAAACT450.1695362242399126No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC450.1695362242399126No Hit
TGCCATCTTGCCACATCTTCTACGGCGGCCAGCTTACTGAGCAGGAAGGG440.16576875259013676No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT400.1506988659910334No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT400.1506988659910334No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAGGGGGCAAACTGAGGT390.14693139434125757No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT390.14693139434125757No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT390.14693139434125757No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT370.1393964510417059No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT360.13562897939193008No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT330.12432656444260257No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC320.12055909279282673No Hit
TGCCATCTTGCCACATCTTCCATGATATATTATAATGACCTATTTGTGCG310.11679162114305089No Hit
TGCCATCTTGCCACATCTTACTAGACGAAAAAAGGGGAAAGGGGGCAAAC290.10925667784349924No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT290.10925667784349924No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT280.1054892061937234No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT270.10172173454394756No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)