FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751
Total Bases833.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT383766.7188315075639No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC5028.728916710137367No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1793.1125021735350376No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1743.025560772039645No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1001.738828029907842No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT300.5216484089723527No Hit
TGCCATCTTGCCACATCTTATGATTCTGCCAAGGAAGAGAGTACTATGGA290.5042601286732742No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA280.48687184837419584No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG260.45209528777603897No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG260.45209528777603897No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC230.39993044687880364No Hit
TGCCATCTTGCCACATCTTAGCTTAAGGGTATCCGCCAACTTGAGGAGAC190.33037732568249No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG160.27821248478525473No Hit
TGCCATCTTGCCACATCTGCACCTAAAATGCCTGAATCAACTCCTGTTTC160.27821248478525473No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT100.1738828029907842No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAAT100.1738828029907842No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT100.1738828029907842No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA90.1564945226917058No Hit
TGCCATCTTGCCACATCTAGTTTAAACTTTTGAGTGGTTTGTGTCTCCTG90.1564945226917058No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA90.1564945226917058Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGT90.1564945226917058No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTGTTTGGTTTCCCAGT80.13910624239262737No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT80.13910624239262737No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT80.13910624239262737No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT80.13910624239262737No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT80.13910624239262737No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC80.13910624239262737No Hit
GAGGACATCTTATGATGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT70.12171796209354896No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT70.12171796209354896No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT70.12171796209354896No Hit
GAGGACATCTTATGGTGCATTGAAATGGAAAGGGTATTTGGTTTCCCAGT70.12171796209354896No Hit
GAGGACATCTTATGGTGCACTGAGATGGAAAGGGTATTTGGTTTCCCAGT70.12171796209354896No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA70.12171796209354896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCTGGTTTCCCAGT70.12171796209354896No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC60.10432968179447052No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGATTTCCCAGT60.10432968179447052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)