FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7219
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT331345.892782933924366No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC125117.329269981991967No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA5858.103615459204876No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT2253.11677517661726No Hit
TGCCATCTTGCCACATCTTTCAGTGAGTAGTAGGAAGAATGTAGGATACC2223.075218174262363No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1952.701205153068292No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1862.5765341460036018No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG530.7341737082698435No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT490.6787643717966477No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG230.31860368472087547No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC200.2770466823659787No Hit
TGCCATCTTGCCACATCTAAACTTACAGTTATGTTTTGCCTCCTGTGAAG190.26319434824767973No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA170.23548968001108186No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC160.22163734589278294No Hit
TGCCATCTTGCCACATCTTAGCAGGGTATCCGCCAACTTGAGGAGACCAA130.18008034353788613No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT120.1662280094195872No Hit
TGCCATCTTGCCACATCTTCGTCTCCGCAGCCTCCTGCAAAGCGGCTGGG120.1662280094195872No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT110.1523756753012883No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG110.1523756753012883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT110.1523756753012883No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT100.13852334118298934No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT100.13852334118298934No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC100.13852334118298934No Hit
GAGGACATCTTGCCACATCTTTCAGTGAGTAGTAGGAAGAATGTAGGATA100.13852334118298934No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC100.13852334118298934No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT90.12467100706469039No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG80.11081867294639147No Hit
GGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC80.11081867294639147No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCAAGGGTATCCGCCAACTTGA80.11081867294639147No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT80.11081867294639147No Hit
TGCCATCTTGCCCATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT80.11081867294639147No Hit
GAGGACATCTTATGGTGCACGCAGAGTACATGGGAAGCAGTGGTATCAAC80.11081867294639147No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGATTTCCCAGT80.11081867294639147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)