FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23053
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1154450.075912028803195No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG410217.793779551468354No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG20628.944605908124755No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC5572.4161714310501887No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2881.2492951025896846No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG2871.2449572723723594No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2110.9152821758556371No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1020.44245868216717993No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA950.41209387064590297No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA890.38606688934195116No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG880.38172905912462585No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG820.3557020778206741No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG790.3426885871686982No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC680.29497245477811995No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.2559319828221923No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.25159415260486706No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.23858066195289115No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.23858066195289115No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT520.2255671713009153No Hit
GTTGGCGGATACCCTTCCCTAAGATGTGGCAAGATGGCAAACTGGTGTAG480.2082158504316141No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA480.2082158504316141No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT470.2038780202142888No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG460.19954018999696352No Hit
TACCTCAGTTTGCCCCCTCTCCCCTTTTTGGCGTCCTAGTAAGATGTGGC450.19520235977963823No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG430.18652669934498764No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.16049971804103585No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA360.15616188782371057No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG330.1431483971717347No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.1388105669544094No Hit
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT300.13013490651975881No Hit
GTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT300.13013490651975881No Hit
GTTGGCGGATACCCTTCCAGAATTTTAGATATTAGAATTAAAATTACTGA280.12145924608510823No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT280.12145924608510823No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG270.11712141586778294No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA260.11278358565045765No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA250.10844575543313235No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.10410792521580706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)