FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3806
Total Bases549.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA150339.49027850761955No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG87322.937467157120338No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1544.046242774566474No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA1012.653704676826064No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC982.5748817656332106No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC892.3384130320546506No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC872.2858644245927486No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA350.9196006305832896No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.7619548081975828No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA230.604308985811876No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT170.44666316342616924No Hit
GTTGGCGGATACCCTTCCATTCTGTTCTTGCCTTCTTTGAAGCAAATAAT160.42038885969521805No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG140.36784025223331585No Hit
GTTGGCGGATACCCTTACACACACGCAAAATACTCCTTCAGCGGAGCGAA120.3152916447714136No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA120.3152916447714136No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.2890173410404624No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG110.2890173410404624No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG110.2890173410404624No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.2890173410404624No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA100.2627430373095113No Hit
GTTGGCGGATACCCTTGCTGAGCCGCATTAGCAGCTGGTAAGATGTGGCA90.2364687335785602No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.2364687335785602No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.2364687335785602No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG80.21019442984760903No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA80.21019442984760903No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA80.21019442984760903No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG80.21019442984760903No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.18392012611665792No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG70.18392012611665792No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCAAACTGGT60.1576458223857068No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.1576458223857068No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA60.1576458223857068No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG50.13137151865475566No Hit
GTTGGCGGATACCCTTGTGGAGGACTGAGAAGATGTGGCAAGATGGCAAA50.13137151865475566No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG50.13137151865475566No Hit
GTTGGCGGATACCCTTCTCTGTGGGGGTGGGAGGTTCCTGAGGAGGAGAG50.13137151865475566No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA50.13137151865475566No Hit
GTTGGCGGATACCCTTCCTTTAGTTTCATATAATTAAAAAAAAAACAGTC50.13137151865475566No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA40.10509721492380451No Hit
GTTGGCGGATACCCTTCTGAGCCAGCATTAGCAGCTGGTACAGATGTGGC40.10509721492380451No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACATACACGCAAAATAC40.10509721492380451No Hit
TACCTCAGTTTGCCCCCGTGCACCATAAGATGTCCTCAACTGGTGTAGAA40.10509721492380451No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG40.10509721492380451No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA40.10509721492380451No Hit
GTTGGCGGATACCCTTGGCCAAAGTGCAGATGTGGCAAGATGGCAAACTG40.10509721492380451No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)