FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9905
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT354335.76981322564362No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA169517.1125694093892No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA139014.033316506814742No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC116811.792024230186774No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3803.8364462392730947No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1371.3831398283695104No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1301.3124684502776376No Hit
TGCCATCTTGCCACATCTCTCAAAGGAATGGAAGGGTATCCGCCAACTTG1061.0701665825340738No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG430.4341241797072186No Hit
TGCCATCTTGCCACATCTTTCAAAGGAATGGAAGGGTATCCGCCAACTTG330.33316506814740027No Hit
TGCCATCTTGCCACATCTCTCAAAGGAATTGAAGGGTATCCGCCAACTTG280.2826855123674912No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT270.2725896012115093No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT190.1918223119636547No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG180.1817264008076729No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT170.17163048965169106No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT160.16153457849570924No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.1514386673397274No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC130.13124684502776374No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT120.12115093387178194No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC120.12115093387178194No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT110.1110550227158001No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT110.1110550227158001No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT110.1110550227158001No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT100.10095911155981827No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG100.10095911155981827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT100.10095911155981827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)