FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11695
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT623453.304831124412146No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC135411.577597263787943No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG6715.737494655835827No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5945.079093629756306No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT720.6156477126977341No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC640.5472424112868748No Hit
TGCCATCTTGCCACATCTTAGTAGTAGCTCTTGTAGGGAGGGGTTGGGGA590.5044890979050877No Hit
TGCCATCTTGCCACATCTTAGCTCTCATTGAAACTGTAAGCAGCCAGGCA550.470286447199658No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG510.43608379649422835No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG430.367678495083369No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT410.3505771697306541No Hit
TGCCATCTTGCCACATCTTAGGTTTACCTGTAAAAAGGATCAAGGATAAT350.2992731936725096No Hit
TGCCATCTTGCCACATCTTAATAGTAGCTCTTGTAGGGAGGGGTTGGGGA330.2821718683197948No Hit
TGCCATCTTGCCACATCTCAAAAAGAAGGAAGGGTATCCGCCAACTTGAG330.2821718683197948No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG270.2308678922616503No Hit
TGCCATCTTGCCACATCTTTTGGTGGAAACTACGAATGTTTAACAATCAC260.22231722958529287No Hit
TGCCATCTTGCCACATCTTGCCAATGGAAATCTGCAGAGTGCCCAGCGAC250.21376656690893547No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC230.1966652415562206No Hit
TGCCATCTTGCCACATCTTAGTCAAATAAAAATTTCAAAATTCTGGTTGG220.1881145788798632No Hit
TGCCATCTTGCCACATCTGGGGTTTACCTGTAAAAAGGATCAAGGATAAT220.1881145788798632No Hit
TGCCATCTTGCCACATCTTCTCTTTCTGCCCTCATCTCTCCTCTCTCCCC220.1881145788798632No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT200.17101325352714836No Hit
TGCCATCTTGCCACATCTGCTCTCTCTGTTCAAGGGTATCCGCCAACTTG190.16246259085079093No Hit
TGCCATCTTGCCACATCTTGGGTTTACCTGTAAAAAGGATCAAGGATAAT190.16246259085079093No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT180.15391192817443353No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT180.15391192817443353No Hit
TGCCATCTTGCCACATCTGAAGAGGAAGGGTATCCGCCAACTTGAGGAGA170.1453612654980761No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT170.1453612654980761No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA160.1368106028217187No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT160.1368106028217187No Hit
GAGGACATCTTATGGTGCACGGGGGCAAACTGAGGTATTGAGGAGACCAA150.12825994014536127No Hit
TGCCATCTTGCCACATCTGTGTATGCATCTGGTTTATTTATTTTAATCAT150.12825994014536127No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA140.11970927746900385No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT140.11970927746900385No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT140.11970927746900385No Hit
TGCCATCTTGCCACATCTTAGCTTTCTTCTCGTCGGTGTTCCCGGCTGCT140.11970927746900385No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC140.11970927746900385No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA140.11970927746900385No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT130.11115861479264644No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT130.11115861479264644No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT120.10260795211628901No Hit
TGCCATCTTGCCACATCTTGGCTTTCTTCTCGTCGGTGTTCCCGGCTGCT120.10260795211628901No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT120.10260795211628901No Hit
TGCCATCTTGCCACATCTTAGCAGGGTATCCGCCAACTTGAGGAGACCAA120.10260795211628901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)