FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11695
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA562048.05472424112869No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG122610.483112441214194No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC6685.7118426678067555No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5734.8995297135528No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA1651.410859341598974No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA710.6070970500213766No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG610.5215904232578025No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.470286447199658No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG510.43608379649422835No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT460.3933304831124412No Hit
GTTGGCGGATACCCTTCCCATTAACCCCTTTAAAATGTAATGCAACTAAC460.3933304831124412No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA430.367678495083369No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT430.367678495083369No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.33347584437793926No Hit
TACCTCAGTTTGCCCCCATGCACTGCCGGGACGCCTCCCAAGCGGCTGCC390.33347584437793926No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.31637451902522445No Hit
GTTGGCGGATACCCTTCCAGGCTTTAACACCCACAGCTTAAAAGCCGTCC370.31637451902522445No Hit
GTTGGCGGATACCCTTCCATAGGCAACCTGGTGGTCCCCCGCTCCCGGGA360.30782385634886705No Hit
GTTGGCGGATACCCTTCCTTCTTTTTGAGATGTGGCAAGATGGCAAACTG310.26507054296707994No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA310.26507054296707994No Hit
GTTGGCGGATACCCTTCCCATTAACCCTTTTAAAATGTAATGCAACTAAC310.26507054296707994No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA310.26507054296707994No Hit
GTTGGCGGATACCCTTCCATGCTTTAACACCCACAGCTTAAAAGCCGTCC290.24796921761436513No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.2394185549380077No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC270.2308678922616503No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG270.2308678922616503No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG270.2308678922616503No Hit
GTTGGCGGATACCCTTCCGCGCGTCCCCTGGGGAAGCCCATCTCGATGAG230.1966652415562206No Hit
GTTGGCGGATACCCTTGAACAGAGAGAGCAGATGTGGCAAGATGGCAAAC220.1881145788798632No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.1881145788798632No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG210.17956391620350579No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA210.17956391620350579No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG210.17956391620350579No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG190.16246259085079093No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA180.15391192817443353No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG170.1453612654980761No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA170.1453612654980761No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGT170.1453612654980761No Hit
TACCTCAGTTTGCCCCCATGATTAAAATAAATAAACCAGATGCATACACA170.1453612654980761No Hit
GTTGGCGGATACCCTTACACACACGCAAAATACTCCTTCAGCGGAGCGAA170.1453612654980761No Hit
GTTGGCGGATACCCTTCCTCTTCAGATGTGGCAAGATGGCAAACTGGTGT170.1453612654980761No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA160.1368106028217187No Hit
GTTGGCGGATACCCTTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA160.1368106028217187No Hit
GTTGGCGGATACCCTTGGCAACTAGTGCATTTGGACTGGAACTACTTGGG160.1368106028217187No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC160.1368106028217187Clontech Universal Primer Mix Long (96% over 26bp)
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG160.1368106028217187No Hit
TACCTCAGTTTGCCCCCGTGCACCATAAGATGTCCTCAACTGGTGTAGAA150.12825994014536127No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC150.12825994014536127No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA150.12825994014536127No Hit
GTTGGCGGATACCCTTCTCGCTGTGATGAGTGATTGTTAAACATTCGTAG150.12825994014536127No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG140.11970927746900385No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA130.11115861479264644No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA130.11115861479264644No Hit
TACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA120.10260795211628901No Hit
GTTGGCGGATACCCTGCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA120.10260795211628901No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA120.10260795211628901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)