FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10699
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT392436.67632489017665No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA372934.85372464716329No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA2312.1590802878773716No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2011.8786802504907No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1661.5515468735395832No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC1531.4300401906720253No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1511.4113468548462473No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGCAGGCATGGGGGCAA960.8972801196373493No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGCAGGCAAGGGGGCGC550.514066735208898No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCACGAGT540.5047200672960089No Hit
TGCCATCTTGCCACATCTTGCTCCTGTCTTCTCACACGTCCACCCTCCAG450.42060005608000745No Hit
TGCCATCTTGCCACATCTTCTTCACATAAAAGTGCAGGTGAAGCAGTGGT390.3645200486026731No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT300.28040003738667163No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT250.23366669782222638No Hit
TGCCATCTTGCCACATCTGTATTTTTCTTTGACTTAGTTGAATTGTATAT250.23366669782222638No Hit
TGCCATCTTGCCACATCTGAAGAGGAAGGGTATCCGCCAACTTGAGGAGA220.20562669408355919No Hit
TGCCATCTTGCCACATCTAGCTCCTGTCTTCTCACACGTCCACCCTCCAG210.19628002617067017No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA210.19628002617067017No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT200.1869333582577811No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC180.16824002243200298No Hit
TGCCATCTTGCCACATCTTGGAAAGGCGAAAAGAACCCCGTCGAGGGGAG140.13085335078044677No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT140.13085335078044677No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA120.11216001495466867No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT120.11216001495466867No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA120.11216001495466867Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)