FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10458
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT588156.234461656148405No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA138213.214763817173456No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC5855.593803786574871No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA5805.545993497800727No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1641.5681774717919297No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1531.4629948364888123No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1471.4056224899598393No Hit
TGCCATCTTGCCACATCTGGAATGGAAGGGTATCCGCCAACTTGAGGAGA1061.013578122011857No Hit
TGCCATCTTGCCACATCTTGAATGGAAGGGTATCCGCCAACTTGAGGAGA900.8605851979345954No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG200.19124115509657677No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC160.15299292407726142No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT160.15299292407726142No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT150.1434308663224326No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC140.13386880856760375No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT140.13386880856760375No Hit
GAGGACATCTTATGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC130.12430675081277491No Hit
TGCCATCTTGCCACATCTGACTTAACAAACCGAAGGGTATCCGCCAACTT130.12430675081277491No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT120.11474469305794606No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT120.11474469305794606No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT120.11474469305794606No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT120.11474469305794606No Hit
GAGGACATCTTATGGCGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT120.11474469305794606No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTGTTTGGTTTCCCAGT110.10518263530311722No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG110.10518263530311722No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT110.10518263530311722No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)