FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14587
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC293120.09323370124083No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA237516.28162062110098No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT199713.690272160142591No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA192113.16926030026736No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT7044.826215123054775No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3312.2691437581408103No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3222.2074449852608486No Hit
TGCCATCTTGCCACATCTTCGGCGCAGAAACCCTTATTTAGACCAGTGAG3072.1046136971275793No Hit
TGCCATCTTGCCACATCTGCCGCATTTTGGCAAGGGGTCCGGGGTCTGGA1461.0008912044971552No Hit
TGCCATCTTGCCACATCTTAGGCGCAGAAACCCTTATTTAGACCAGTGAG1110.7609515321861932No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1100.7540961129773086No Hit
TGCCATCTTGCCACATCTCAATGTATCGTTCTTAACCCCACCTCCTGTAA730.5004456022485776No Hit
TGCCATCTTGCCACATCTCTGCCCATTAATTGGAGACTTTACAACATTTA720.4935901830396929No Hit
TGCCATCTTGCCACATCTTCCATGATATATTATAATGACCTATTTGTGCG640.4387468293686159No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG600.4113251525330774No Hit
TGCCATCTTGCCACATCTTGTCCTCTTACTTCTCACCCGCCTGCACCACA480.3290601220264619No Hit
TGCCATCTTGCCACATCTTAGGTCTTGTAGTCTATAATGGAAAGGCTAAA420.28792760677315415No Hit
TGCCATCTTGCCACATCTTCACTAATTGCTTCCAAAAGCAGTGGTATCAA410.28107218756426955No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC370.25365051072873107No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT330.2262288338931926No Hit
TGCCATCTTGCCACATCTTGTTTGGGGAAAAATCAGCCATCTATAAGCAG310.21251799547542333No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.19195173784876945No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT260.17824089943100022No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.17824089943100022No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG260.17824089943100022No Hit
TGCCATCTTGCCACATCTGCAGTGTTTTGATGCTGCCCTCTATCTACAAA260.17824089943100022No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.17138548022211558No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT240.16453006101323095No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC240.16453006101323095No Hit
TGCCATCTTGCCACATCTTAGCTCTAATGCTGGTTCAGCAAGGGTATCCG220.1508192225954617No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT210.14396380338657708No Hit
TGCCATCTTGCCACATCTCAAGCCAAGCAGTGGTATCAACGCAGAGTACA200.13710838417769244Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT180.12339754575992322No Hit
TGCCATCTTGCCACATCTTTCTTCCCCGTCATCAGAGACAGCAAAGAGGA180.12339754575992322No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT180.12339754575992322No Hit
TGCCATCTTGCCACATCTAACAACACATTGCCCTTTGTGTTATGTGATCA180.12339754575992322No Hit
TGCCATCTTGCCACATCTTCCGCATTTTGGCAAGGGGTCCGGGGTCTGGA170.1165421265510386No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA160.10968670734215398No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT160.10968670734215398No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGTTGA160.10968670734215398No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC160.10968670734215398No Hit
TGCCATCTTGCCACATCTAGTTTTATTAGAAGTTGACTAAGAATATTACT160.10968670734215398No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT160.10968670734215398No Hit
TGCCATCTTGCCACATCTCTTAGTGGTCCCTCTACCTGAAAAACCTTCTT150.10283128813326935No Hit
TGCCATCTTGCCACATCTACTGAAGCAGTGGTATCAACGCAGAGTACATG150.10283128813326935Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.10283128813326935No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCAAGGGTATCCGCCAACTTGA150.10283128813326935No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT150.10283128813326935No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.10283128813326935No Hit
GAGGACATCTTGCCACATCTTCGGCGCAGAAACCCTTATTTAGACCAGTG150.10283128813326935No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)