FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871
Total Bases413.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA124643.39951236502961No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC55719.40090560780216No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC36612.748171368861025No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1204.179728317659352No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC531.8460466736328804No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC511.7763845350052248No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT190.6617903169627307No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG80.2786485545106235No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG80.2786485545106235No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.2786485545106235No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG70.24381748519679552No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.20898641588296762No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT60.20898641588296762No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.20898641588296762No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.20898641588296762No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.17415534656913967No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG50.17415534656913967No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT40.13932427725531174No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC40.13932427725531174No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGGCCTTAGCTCAGCAC40.13932427725531174No Hit
GTTGGCGGATACCCTTGAATCCCTAACACTGCGGTGTAACGGAGGTCTCA40.13932427725531174No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT40.13932427725531174No Hit
TACCTCAGTTTGCCCCAGTGCACCATAAGATGTCCTCAACTGGTGTAGAA40.13932427725531174No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG40.13932427725531174No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.13932427725531174No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCCCCCTGGACCTTAGCTCAGCAC30.10449320794148381No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA30.10449320794148381No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT30.10449320794148381No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC30.10449320794148381No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCCGCAC30.10449320794148381No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG30.10449320794148381No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA30.10449320794148381No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTACTCCTTCA30.10449320794148381No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG30.10449320794148381No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG30.10449320794148381No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA30.10449320794148381No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT30.10449320794148381No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGCTCCTTCA30.10449320794148381No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA30.10449320794148381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)