FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34189
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1815453.09894995466378No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG740021.644388546023574No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC9492.775746585158969No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC4551.3308374038433415No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3871.1319430226096112No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA3370.9856971540553979No Hit
GTTGGCGGATACCCTTCCTCTACGCCTAAGGTCACCGCAGTAAACAAAGG2950.8628506244698587No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1830.5352598789084209No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1730.5060107051975782No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA1580.4621369446313142No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1530.4475123577758928No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1150.3363654976746907No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1100.32174091081926937No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1080.3158910760771008No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1000.2924917371084267No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA920.26909239813975255No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA910.2661674807686683No Hit
TACCTCAGTTTGCCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGG910.2661674807686683No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA860.2515428939132469No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG840.24569305917107842No Hit
TACCTCAGTTTGCCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGA740.21644388546023574No Hit
TACCTCAGTTTGCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGGC700.20474421597589867No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG680.19889438123373016No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG610.17841995963614027No Hit
GTTGGCGGATACCCTTCCTGGGGCCGTCTATTAGGGACCTCGCCCGAGAG610.17841995963614027No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.17257012489397175No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.16087045540963468No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA530.15502062066746614No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA510.14917078592529762No Hit
TACCTCAGTTTGCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGATG500.14624586855421334No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG490.1433209511831291No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG480.14039603381204482No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA470.13747111644096055No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT430.12577144695662346No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA370.10822194273011787No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA360.10529702535903361No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA350.10237210798794934No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA350.10237210798794934No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)