FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17380
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT903051.956271576524735No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC232213.36018411967779No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA9455.43728423475259No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5723.2911392405063293No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5373.0897583429229No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3552.042577675489068No Hit
TGCCATCTTGCCACATCTTTTCCCACTTAACCATGACTTTGGGAAGGGTA2941.6915995397008055No Hit
TGCCATCTTGCCACATCTTACTTCAGTGAAATTAAGTACCAAATTCCAGT2751.5822784810126582No Hit
TGCCATCTTGCCACATCTTAGTGTGCAGTTTAGTAGTGTTAAGCCTCTAG1130.6501726121979287No Hit
TGCCATCTTGCCACATCTTCCCTGTTAGGTACTGCTCCTGTTTCTCTCCT580.33371691599539705No Hit
TGCCATCTTGCCACATCTCTAAAATTGGACAGGAGCCAAAAATGTGGCAG570.32796317606444186No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG570.32796317606444186No Hit
TGCCATCTTGCCACATCTGGAATGGAAGGGTATCCGCCAACTTGAGGAGA530.3049482163406214No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGTCT470.27042577675489066No Hit
TGCCATCTTGCCACATCTTGAATGGAAGGGTATCCGCCAACTTGAGGAGA470.27042577675489066No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT460.2646720368239356No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC450.2589182968929804No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG320.18411967779056385No Hit
TGCCATCTTGCCACATCTTAGACAAATGAGCAAAGCAGTGTGTGGGCCAA300.17261219792865362No Hit
TGCCATCTTGCCACATCTGTTAATACGCTAACCGCACCAGCATGGGGGCA290.16685845799769852No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG270.15535097813578827No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT250.14384349827387802No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA230.1323360184119678No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT220.12658227848101267No Hit
TGCCATCTTGCCACATCTCATCCGCTAATTTTTCAACATTAGTCGGTTCG220.12658227848101267No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT210.12082853855005754No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC210.12082853855005754No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT210.12082853855005754No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT210.12082853855005754No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT210.12082853855005754No Hit
TGCCATCTTGCCACATCTTTTCCCACTTAACTAGGAATTTGGGGCCTTAG200.11507479861910241No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC190.10932105868814729No Hit
TGCCATCTTGCCACATCTTACTTCAGTGAAATTAAGTACCAAATTCCAAT190.10932105868814729No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT190.10932105868814729No Hit
TGCCATCTTGCCACATCTGTTCTGTTGAAGATGGAAGGGTATCCGCCAAC190.10932105868814729No Hit
TGCCATCTTGCCACATCTATTGTGTAGTTATTTAACAGATTAAATAGAAG180.10356731875719218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)