FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17380
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA863049.65477560414269No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG245014.096662830840046No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC9165.27042577675489No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5723.2911392405063293No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5363.084004602991945No Hit
GTTGGCGGATACCCTTCCCAAAGTCATGGTTAAGTGGGAAAAGATGTGGC2551.467203682393556No Hit
GTTGGCGGATACCCTTCCACTGGAATTTGGTACTTAATTTCACTGAAGTA1200.6904487917146145No Hit
GTTGGCGGATACCCTTCCTGAAGATCAGTGTTTTCCCATCAACTCTGCCA1050.6041426927502876No Hit
GTTGGCGGATACCCTTGCCACTGGAATTTGGTACTTAATTTCACTGAAGT920.5293440736478712No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG730.42002301495972383No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA690.3970080552359033No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA670.3855005753739931No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG610.35097813578826237No Hit
GTTGGCGGATACCCTTCCATTCCAGATGTGGCAAGATGGCAAACTGGTGT530.3049482163406214No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.3049482163406214No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.2991944764096663No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG510.29344073647871116No Hit
GTTGGCGGATACCCTTGGGAAGTTTGGCTATAAAGGGAGGAGAGAAACAG490.2819332566168009No Hit
GTTGGCGGATACCCTTCCATTCAAGATGTGGCAAGATGGCAAACTGGTGT460.2646720368239356No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA460.2646720368239356No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG440.25316455696202533No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG440.25316455696202533No Hit
GTTGGCGGATACCCTTGCAACAACCCAAGAAGGCTGCCACATTTTTGGCT420.24165707710011508No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT370.21288837744533948No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.1956271576524741No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG330.18987341772151897No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG320.18411967779056385No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG310.17836593785960875No Hit
GTTGGCGGATACCCTTCCCACTGGAATTTGGTACTTAATTTCACTGAAGT310.17836593785960875No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.16685845799769852No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.16685845799769852No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC270.15535097813578827No Hit
TACCTCAGTTTGCCCCCATGCTGGTGCGGTTAGCGTATTAACAGATGTGG270.15535097813578827No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG260.14959723820483314No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG250.14384349827387802No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGT250.14384349827387802No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA240.1380897583429229No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG230.1323360184119678No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA220.12658227848101267No Hit
GTTGGCGGATACCCTTGGTTGAAGGTTGGGTAACACTAACTGGAGGACCG210.12082853855005754No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG210.12082853855005754No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA210.12082853855005754No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA210.12082853855005754No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA200.11507479861910241No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA190.10932105868814729No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGAACAGATGTGGCAAGATGGCA190.10932105868814729No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)