FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150
Total Bases456.1 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA128640.82539682539682No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA43113.682539682539682No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1745.523809523809524No Hit
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGTAT1003.1746031746031744Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG973.0793650793650795No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGTAT712.253968253968254Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG702.2222222222222223No Hit
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGAAG391.2380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGTAT361.1428571428571428Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGAAG290.9206349206349206Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC180.5714285714285714No Hit
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGAAG150.4761904761904762Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGGTG120.38095238095238093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGT110.3492063492063492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT100.31746031746031744No Hit
TGCCATCTTGCCACATCTTGTTCTGCTCGTAAGAAGCAGTGGTATCAACG100.31746031746031744No Hit
GAGGACATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGT80.25396825396825395Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGGAA70.2222222222222222Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTACAGTGGTATCAACGCAGAGTACATGGGTAT70.2222222222222222Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGCCATCTTGCCACATCTTGTTGTCCAGCACAAAGCAGTGGTATCAACGC70.2222222222222222No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGGTG70.2222222222222222Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT70.2222222222222222No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT60.19047619047619047No Hit
TGCCATCTTGCCACATCTTCCCAAGGGTATCCGCCAACTTGAGGAGACCA60.19047619047619047No Hit
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGGTG60.19047619047619047Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGGACATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGA60.19047619047619047Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGG60.19047619047619047Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGTGT50.15873015873015872Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG50.15873015873015872No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG50.15873015873015872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGGGG50.15873015873015872Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA50.15873015873015872No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG50.15873015873015872No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGGTA50.15873015873015872Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.12698412698412698No Hit
TGCCATCTTGCCACATCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT40.12698412698412698No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.12698412698412698No Hit
TGCCATCTTGCCACATCTATACTGGCTTTTTCAAATTCTTCCTTCTCCTT40.12698412698412698No Hit
GAGGACATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGA40.12698412698412698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTCCAAAGCAGTGGTATCAACGCAGAGTACATG40.12698412698412698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGACG40.12698412698412698Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTTGCTCGAATC40.12698412698412698Clontech SMARTer II A Oligonucleotide (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)