FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656
Total Bases384.8 kbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC97736.78463855421687No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG56721.34789156626506No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC38214.38253012048193No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC521.957831325301205No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC451.694277108433735No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT180.677710843373494No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT160.6024096385542169No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG120.4518072289156626No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA100.37650602409638556No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG100.37650602409638556No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG90.338855421686747No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA90.338855421686747No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA80.30120481927710846No Hit
GTTGGCGGATACCCTTCCATGGAAGTTTTCAGAGATGTGGCAAGATGGCA70.2635542168674699No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.2635542168674699No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT70.2635542168674699No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.2635542168674699No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.2635542168674699No Hit
GTTGGCGGATACCCTTGGGCTGGTTTCACCCTGGAGATGTGGCAAGATGG70.2635542168674699No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA60.2259036144578313No Hit
GTTGGCGGATACCCTTCCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG50.18825301204819278No Hit
GTTGGCGGATACCCTTCCACGGAAGTTTTCAGAGATGTGGCAAGATGGCA50.18825301204819278No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT50.18825301204819278No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.18825301204819278No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG50.18825301204819278No Hit
GTTGGCGGATACCCTTGGGTGAGATGTGGCAAGATGGCAAACTGGTGTAG40.15060240963855423No Hit
TACCTCAGTTTGCCCCCATGGGCTCGTGTCGCCGGTGTAGCCCCCGCGGT40.15060240963855423No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT40.15060240963855423No Hit
GTTGGCGGATACACTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG40.15060240963855423No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA40.15060240963855423No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT40.15060240963855423No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA30.11295180722891565No Hit
GTTGGCGGATACCCTTCCTTGTGAATTTATCTGCTTGCTAAAATTTATTT30.11295180722891565No Hit
GTTGGCGGATACCCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCA30.11295180722891565No Hit
GTTGGCGGATACCCTTCATTGTGAATTTATCTGCTTGCTAAAATTTATTT30.11295180722891565No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC30.11295180722891565No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC30.11295180722891565No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA30.11295180722891565No Hit
GTTGGCGGATACCCTTCCCAGAGATGTGGCAAGATGGCAAACTGGTGTAG30.11295180722891565No Hit
GTGTCGCTACCTCAGTTTGCACACTGACCACATCACCATCCACCTTAACT30.11295180722891565No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.11295180722891565Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG30.11295180722891565No Hit
GTTGGCGGATACCCTTCCATGTTGGCGGATACCCTTCTGTGTTGGCGGAT30.11295180722891565No Hit
GTTGGCGGATACCCTGCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA30.11295180722891565No Hit
GTTGGCGGATACCCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAAT30.11295180722891565No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG30.11295180722891565No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC30.11295180722891565No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT30.11295180722891565No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)