FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241
Total Bases324.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC73132.61936635430611No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG32714.591700133868809No Hit
GTTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGG22610.084783578759481No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC693.0789825970548863No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC522.3203926818384653No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT321.42793395805444No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT210.9370816599732262No Hit
GTTGGCGGATACCCTTCCTGATGCGTTAGTTCCCAGGTGGAGCTGCATGT210.9370816599732262No Hit
GTTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGTCCTCAACT170.7585899152164213No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT140.6247211066488175No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT120.535475234270415No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.4908522980812137No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA110.4908522980812137No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA100.4462293618920125No Hit
TACCTCAGTTTGCCCCCTGGTCTTCTGGAGAAAAGATGTGGCAAGATGGC100.4462293618920125No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG100.4462293618920125No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.35698348951361No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA80.35698348951361No Hit
GTTGGCGGATACCCTTCCATCTTCTAAGATGTGGCAAGATGGCAAACTGG80.35698348951361No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA80.35698348951361No Hit
GTTGGCGGATACCCTTCCAACTTCTTAGATGTGGCAAGATGGCAAACTGG70.31236055332440876No Hit
GTTGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGGT60.2677376171352075No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA60.2677376171352075No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC60.2677376171352075Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTGCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA60.2677376171352075No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA50.22311468094600626No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA50.22311468094600626No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG50.22311468094600626No Hit
GTTGGCGGATACCCTTGGCAAGATGTGGCAAGATGGCAAACTGGTGTAGA40.178491744756805No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.178491744756805No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGGCCTTAGCTCAGCAC40.178491744756805No Hit
GTTGGCGGATACCCTTCCAACTTCTAAGATGTGGTTAGACGGCAAACTGG40.178491744756805No Hit
GTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGGT40.178491744756805No Hit
GTTGGGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGGT40.178491744756805No Hit
GTTGGCGGATACCCTTCCGGAATCTGTCGCAGACAAGATGAAGATGTGGC40.178491744756805No Hit
GTTGGCGGATACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATA40.178491744756805No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC40.178491744756805No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT40.178491744756805No Hit
GTTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGCAAACTGGT40.178491744756805No Hit
GTTGGCGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGGT40.178491744756805No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT30.13386880856760375No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA30.13386880856760375No Hit
CGAAGTAGACCAGCGGCATCTCGACCCCACCCTGGACCTTAGCTCAGCAC30.13386880856760375No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA30.13386880856760375No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT30.13386880856760375No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG30.13386880856760375No Hit
TTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGGT30.13386880856760375No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCGGTGTTGGCGGAT30.13386880856760375No Hit
GTTGGCGGATACCCTTGAGGAAGAGAAAATAATAATGATTTCCTAAGATG30.13386880856760375No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCCC30.13386880856760375No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAT30.13386880856760375No Hit
GTTGGCGGATACCCTTCCATGTTGGCGGATACCCTTCTGTGTTGGCGGAT30.13386880856760375No Hit
GTTGGCGGATACCCTAGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT30.13386880856760375No Hit
GTTGGCGGATACCCTTGGCGGAGATGTGGCAAGATGGCAAACTGGTGTAG30.13386880856760375No Hit
GTTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAACTGGT30.13386880856760375No Hit
GTTGGCGGATACCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGGT30.13386880856760375No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA30.13386880856760375No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT30.13386880856760375No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT30.13386880856760375No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT30.13386880856760375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)