FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909
Total Bases418 kbp
Sequences flagged as poor quality0
Sequence length35-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA161555.51735991749742No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG40013.750429700928155No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC913.1282227569611547No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT381.3062908215881746No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC311.0656583018219319No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC301.0312822275696116No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGATGTGGCA290.9969061533172912Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC240.8250257820556892No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG170.5843932622894465No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC120.4125128910278446Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA120.4125128910278446No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG120.4125128910278446No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG120.4125128910278446No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG100.3437607425232038No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.30938466827088346No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.30938466827088346No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.24063251976624267No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.24063251976624267No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATACTCCTTCA70.24063251976624267No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.2062564455139223No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.2062564455139223No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA60.2062564455139223No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA60.2062564455139223No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA60.2062564455139223No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA60.2062564455139223No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTG60.2062564455139223Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAGAATACTCCTTCA50.1718803712616019No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA50.1718803712616019No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA50.1718803712616019No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA50.1718803712616019No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT50.1718803712616019No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCCATGTACTCTGCGTTG40.13750429700928155No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTACA40.13750429700928155No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG40.13750429700928155No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAA40.13750429700928155No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA40.13750429700928155No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACCCCATGTACTCTGC40.13750429700928155Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA40.13750429700928155No Hit
TACCTCAGATTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA40.13750429700928155No Hit
TACCTCAGTTTGCCCCCATGTCCCTTGCACACACGCAAAATACTCCTTCA40.13750429700928155No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG40.13750429700928155No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.13750429700928155No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA30.10312822275696115No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG30.10312822275696115No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT30.10312822275696115Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.10312822275696115Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA30.10312822275696115No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACCCCATGTACTCTGC30.10312822275696115Clontech SMART CDS Primer II A (95% over 21bp)
TACCTCAGTTTGCCCCCATGTCCCTTACACACGCGCAAAATACTCCTTCA30.10312822275696115No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACGCACGCAAAATACTCCTTCA30.10312822275696115No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTGTACTCTGCGTTGA30.10312822275696115No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA30.10312822275696115No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG30.10312822275696115No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)