FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25513
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT687426.943127033277154No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC471818.492533218359267No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG269010.543644416571944No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA21978.611296201936268No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT18667.3139183945439585No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA12404.860267314702309No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG4201.6462195743346528No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3341.3091365186375574No Hit
TGCCATCTTGCCACATCTTTTGGATGGAAGGGTATCCGCCAACTTGAGGA3271.2816995257319799No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2530.9916513150158743No Hit
TGCCATCTTGCCACATCTTTTGGATTGAAGGGTATCCGCCAACTTGAGGA1390.5448202876964685No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA950.3723591894328382No Hit
TGCCATCTTGCCACATCTTTTGGAAGCAAGGGTATCCGCCAACTTGAGGA870.3410026261121781No Hit
TGCCATCTTGCCACATCTTAGGATGGAAGGGTATCCGCCAACTTGAGGAG840.3292439148669306No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA790.3096460627915181No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG790.3096460627915181No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACTTGAGGA740.2900482107161055No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT610.23909379532003291No Hit
TGCCATCTTGCCACATCTCTAGCAATAAACTGGCCCACCAACTGTGGTGC600.2351742249049504No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT570.2234155136597029No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT460.1803002390937953No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC420.1646219574334653No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCCCAACTTGAGGA410.1607023870183828No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.1607023870183828No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCACTTGAGGA400.15678281660330026No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACTTGAGGA390.15286324618821778No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC380.14894367577313528No Hit
TGCCATCTTGCCACATCTTAGGATTGAAGGGTATCCGCCAACTTGAGGAG370.14502410535805274No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.14110453494297026No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT360.14110453494297026No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGTATCCGCCAACTTGAGGA350.13718496452788775No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACTTGAG320.12542625328264023No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAATTGAGGA300.1175871124524752No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT300.1175871124524752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)