FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25513
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA663426.00243013365735No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG597923.43511151177831No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG261610.253596205855839No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC20508.035119350919139No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC12194.777956335985576No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG4111.6109434405989103No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3291.2895386665621449No Hit
GTTGGCGGATACCCTTCCATCCAAAAGATGTGGCAAGATGGCAAACTGGT3211.2581821032414846No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2470.9681338925253792No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1450.5683377101869636No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1420.556578998941716No Hit
GTTGGCGGATACCCTTCAATCCAAAAGATGTGGCAAGATGGCAAACTGGT1400.548739858111551No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1070.4193940344138282No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1050.4115548935836632No Hit
GTTGGCGGATACCCTTGCTTCCAAAAGATGTGGCAAGATGGCAAACTGGT880.3449221965272606No Hit
GTTGGCGGATACCCTTCCATCCTAAGATGTGGCAAGATGGCAAACTGGTG840.3292439148669306No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA760.29788735154627055No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT760.29788735154627055No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT750.293967781131188No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA730.286128640301023No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA700.2743699290557755No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT600.2351742249049504No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.22733508407478542No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.21557637282953787No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT470.1842198095088778No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.1842198095088778No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG450.17638066867871283No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.17638066867871283No Hit
GTTGGGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT410.1607023870183828No Hit
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT390.15286324618821778No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.15286324618821778No Hit
GTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT390.15286324618821778No Hit
GTTGGCGGATACCCTTCAATCCTAAGATGTGGCAAGATGGCAAACTGGTG370.14502410535805274No Hit
GTTGGCGGATACCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT340.13326539411280522No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA340.13326539411280522No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG320.12542625328264023No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.12542625328264023No Hit
GTTGGCGGATACCCTTCGCATTGGAGGGGAAGGCTAGTCATAGAGAGATG310.12150668286755771No Hit
TTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT290.11366754203739271No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG260.10190883079214519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)