FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6024
Total Bases867 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA341256.640106241699876No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG76912.765604249667994No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC3325.511288180610889No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1622.689243027888446No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1191.9754316069057105No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1141.8924302788844622No Hit
GTTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTG310.5146082337317397No Hit
GTTGGCGGATACCCTTCCCCCTCTTCTGGAGTTGTCAAATGACTTGTTAC300.49800796812749004No Hit
GTTGGCGGATACCCTTCCAGTGAAAGTGTGCCTGTTATAGCAGCACAAGG280.46480743691899074No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG250.41500664010624166No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.398406374501992No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG230.38180610889774236No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA230.38180610889774236No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG220.36520584329349265No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA190.3154050464807437No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG190.3154050464807437No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA170.2822045152722444No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG150.24900398406374502No Hit
GTTGGCGGATACCCTTCCATTCTGTTCTTGCCTTCTTTGAAGCAAATAAT150.24900398406374502No Hit
GTTGGCGGATACCCTTCTCCAGGAGTTATGACCTGTTTTTGAGGGGACAC140.23240371845949537No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA110.18260292164674632No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG110.18260292164674632No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA100.16600265604249667No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACCCGCAAAATACTCCTTCA90.14940239043824702No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.14940239043824702No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG80.13280212483399734No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG70.11620185922974768No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGCTCCTTCA70.11620185922974768No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA70.11620185922974768No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.11620185922974768No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA70.11620185922974768No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG70.11620185922974768No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCATTCA70.11620185922974768No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG70.11620185922974768No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG70.11620185922974768No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)