FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15208
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT734548.296948974224094No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC294419.35823250920568No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG10056.608364018937402No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4753.123356128353498No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA4052.6630720673329824No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3592.360599684376644No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1531.0060494476591268No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG1430.940294581799053No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG760.4997369805365597No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC450.2958968963703314No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA370.24329300368227247No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACTTGAG290.19068911099421357No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT290.19068911099421357No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA280.1841136244082062No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACTTGAGGA280.1841136244082062No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT280.1841136244082062No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT270.17753813782219885No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT270.17753813782219885No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA260.17096265123619148No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT240.15781167806417673No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT230.15123619147816939No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG230.15123619147816939No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT230.15123619147816939No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT220.144660704892162No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA190.12493424513413992No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT190.12493424513413992No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC180.11835875854813256No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACTTGAGGA160.10520778537611783No Hit
TGCCATCTTGCCACATCTTAGCAGGGTATCCGCCAACTTGAGGAGACCAA160.10520778537611783No Hit
TGCCATCTTGCCACATCTTGCGACCTCAGATCAGACGTGGCGACCCGCTG160.10520778537611783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)