FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15208
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA692045.50236717517096No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG284618.71383482377696No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG9536.266438716465019No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC4623.0378748027354026No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC3772.4789584429247764No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3332.1896370331404524No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG1340.8811152025249869No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA830.5457653866386113No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG660.43398211467648606No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.3945291951604419No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG570.37480273540241976No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.34192530247238295No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT470.30904786954234614No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG440.289321409784324No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG420.2761704366123093No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA400.2630194634402946No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.2564439768542872No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.2564439768542872No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA350.23014203051025775No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA330.21699105733824303No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.21041557075223566No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT280.1841136244082062No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG270.17753813782219885No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG260.17096265123619148No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG260.17096265123619148No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA250.1643871646501841No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA240.15781167806417673No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT230.15123619147816939No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT230.15123619147816939No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC210.13808521830615464No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG200.1315097317201473No Hit
GTTGGCGGATACCCTTCTCCTCCGCTGACTAATATGCTTAAATTCAGCGG200.1315097317201473No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA200.1315097317201473No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA190.12493424513413992No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA190.12493424513413992No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA160.10520778537611783No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG160.10520778537611783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)