FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55301
Total Bases8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2247140.63398491889839No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA996018.010524221985136No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA727613.157085766984322No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC51169.25118894775863No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT18033.260338872714779No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5380.9728576336775103No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4660.8426610730366539No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT2370.4285636787761523No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACACGGTC2090.3779316829713748No Hit
TGCCATCTTGCCACATCTTTCAGCTCCTGTTATTGGTCTGTTCAGAGAGT1950.3526156850689861No Hit
TGCCATCTTGCCACATCTGTTTGTATTCTTCTTCTGCTCAGCCTCTGGCT1400.2531599790238875No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT970.17540369975226489No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT810.14647113072096346No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC800.1446628451565071No Hit
TGCCATCTTGCCACATCTTGATGTTTGTGTGTACCTCTGCAGCTGCTTTC770.1392379884631381No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCAACTA730.13200484620531275No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG730.13200484620531275No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT700.12657998951194374No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT650.11753856168966204No Hit
TGCCATCTTGCCACATCTCTACAACTATCTGATCTTTGACAAACCTGACA640.11573027612520569No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT570.10307227717401132No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT560.10126399160955497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)