FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411
Total Bases204.3 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA42530.120481927710845No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1057.44153082919915No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG976.874557051736358No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG795.598866052445074No Hit
TGCCATCTTGCCACATCTAGGAAGGGTATCCGCCAACTTGAGGAGACCAA392.7639971651311126No Hit
TGCCATCTTGCCACATCTTCCTTCAGCAAGCAGTGGTATCAACGCAGAGT342.4096385542168677Clontech Universal Primer Mix Long (100% over 25bp)
TGCCATCTTGCCACATCTTGGAAGGGTATCCGCCAACTTGAGGAGACCAA332.3387668320340187No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT191.3465627214741318No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC171.2048192771084338No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG140.9922041105598866Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGAAGAGGAAGGGTATCCGCCAACTTGAGGAGA90.6378454996456413No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA80.5669737774627923No Hit
TGCCATCTTGCCACATCTTGTGCCAATGTCGGAAGGGTATCCGCCAACTT80.5669737774627923No Hit
TGCCATCTTGCCACATCTTCCTCCCTGAAGCAGTGGTATCAACGCAGAGT80.5669737774627923Clontech Universal Primer Mix Long (100% over 23bp)
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA70.4961020552799433No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA70.4961020552799433No Hit
TGCCATCTTGCCACATCTTCCCCCACTCCCCAAGGGTATCCGCCAACTTG70.4961020552799433No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA70.4961020552799433No Hit
TGCCATCTTGCCACATCTTATTTCTGGGCAAGCAGTGGTATCAACGCAGA70.4961020552799433Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTCATTTCTGGGCAAGCAGTGGTATCAACGCAGA60.42523033309709424Clontech Universal Primer Mix Long (100% over 25bp)
TGCCATCTTGCCACATCTTCCCACATGGGAAGCAGTGGTATCAACGCAGA60.42523033309709424Clontech Universal Primer Mix Long (100% over 21bp)
TGCCATCTTGCCACATCTTAGAATTATCACTGAGAAGCAGTGGTATCAAC50.3543586109142452No Hit
TGCCATCTTGCCACATCTTCGTCTCTCAGAAGGCAGGGGAAGAAGCAGTG50.3543586109142452No Hit
TGCCATCTTGCCACATCTGCAATCCTCAAGGGTATCCGCCAACTTGAGGA50.3543586109142452No Hit
TGCCATCTTGCCACATCTTGGAAGGGTATCCGCCAACACAGAAGGGTATC50.3543586109142452No Hit
TGCCATCTTGCCACATCTTAGTCTCTCAGAAGGCAGGGGAAGAAGCAGTG50.3543586109142452No Hit
TGCCATCTTGCCACATCTCTCCAAGCAGTGGTATCAACGCAGAGTACATG50.3543586109142452Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCGAAGCAGTGGTATCAACGCAGAGTACAT40.28348688873139616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCGTCTAACCACATCTTCCTTCAGCAAGCAGTGGTATCAACGCAGAGT40.28348688873139616Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTTGTACCAGCAAGGGTATCCGCCAACTTGAGGA40.28348688873139616No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA40.28348688873139616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACGGGGGCAAACTGAGGTATTGAGGAGACCAA40.28348688873139616No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC40.28348688873139616No Hit
TGCCATCTTGCCACATCTTTTCCCCAGCGCGATGGAAGGGTATCCGCCAA30.21261516654854712No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC30.21261516654854712No Hit
TGCCATCTTGCCACATCTTATCGCAGGCTACCACGTCCTTCATCGCCTTC30.21261516654854712No Hit
TGCCATCTTGCCACATCTTAGAGACCCCTGCCCAGCCCCAACTCCATGCC30.21261516654854712No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA30.21261516654854712Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT30.21261516654854712No Hit
TGCCATCTTGCCACATCTTACACCAGCCAGCCCCAAGGGGCCGTGTCCTT30.21261516654854712No Hit
TGCCATCTTGCCACATCTTGAGCGGCCGAAGGGTATCCGCCAACTTGAGG30.21261516654854712No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG30.21261516654854712No Hit
TGCCATCTTGCCACATCTAGGAAGGGTATCCGCCAACACAGAAGGGTATC30.21261516654854712No Hit
TGCCATCTTGCCACATCTGACACCAGCCAGCCCCAAGGGGCCGTGTCCTT30.21261516654854712No Hit
GAGGACATCTTATGGTGCACTAAGCAGTGGTATCAACGCAGAGTACATGG30.21261516654854712Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGGAAGGGTATCCGCCAACTTGAGGAGACCA30.21261516654854712No Hit
TGCCATCTTGCCACATCTTTCTCTCCCCCGCTCCCCGTCCTCCCCCCTCC30.21261516654854712No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT30.21261516654854712No Hit
TGCCATCTTGCCACATCTGCTCACACATGGGAAGCAGTGGTATCAACGCA30.21261516654854712No Hit
TGCCATCTTGCCACATCTTAGGTATCCGCCAACACAGCCTGTGAAGCAGT20.14174344436569808No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGGTTCCCAGTCCACTA20.14174344436569808No Hit
TGCCATCTTGCCACATCTTGTGCCAATGTCGTAAGGGTATCCGCCAACTT20.14174344436569808No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC20.14174344436569808No Hit
TGCCATCTTGCCACATCTTGTTGACCTCAAAGCAGTGGTATCAACGCAGA20.14174344436569808Clontech Universal Primer Mix Long (100% over 21bp)
TGCCATCTTGCCACATCTTAGCCCAAGCAGTGGTATCAACGCAGAGTACA20.14174344436569808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTGCCACATCTTGGAAGGGTATCCGCCAACTTGAGGAGACC20.14174344436569808No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACTTGAGGAGACCAAGTCTC20.14174344436569808No Hit
GAGGACATCTTATGGTGCACTAAAGCAGTGGTATCAACGCAGAGTACATG20.14174344436569808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCATCTTGCCACATCTTCCTTCAGCAAGCAGTGGTATCAACGCAGAGTA20.14174344436569808Clontech Universal Primer Mix Long (100% over 25bp)
TGCCATCTTGCCACATCTCCCTCCCCAAGGGTATCCGCCAACTTGAGGAG20.14174344436569808No Hit
TGCCATCTTGCCACATCTTTTCCCCAGCGCGGTGGAAGGGTATCCGCCAA20.14174344436569808No Hit
TGCCATCTTGCCACATCTTCCTTCAGTAAGCAGTGGTATCAACGCAGAGT20.14174344436569808Clontech Universal Primer Mix Long (96% over 25bp)
GAGGACATCTTGCCACATCTTCCCCCACTCCCCAAGGGTATCCGCCAACT20.14174344436569808No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG20.14174344436569808No Hit
TGCCATCTTGCCACATCTTTTCGCAGCTTACCAAAGGGTATCCGCCAACA20.14174344436569808No Hit
TGCCATCTTGCCACATCTTGCAAGGGTATCCGCCAACTTGAGGAGACCAA20.14174344436569808No Hit
TGCCATCTTGCCACATCTTTCTGATTGCTGGAAAGCAGTGGTATCAACGC20.14174344436569808No Hit
TGCCATCTTGCCACATCTTAGGTGCCCTTCCGTCAAGGGTATCCGCCAAC20.14174344436569808No Hit
TGCCATCTTGCCACATCTTCCGCGCCCCGCCAAGGGTATCCGCCAACACA20.14174344436569808No Hit
GAGGACATCTTGCCACATCTTCCTTCAGCAAGCAGTGGTATCAACGCAGA20.14174344436569808Clontech Universal Primer Mix Long (100% over 23bp)
GAGGACATCTTATGGTGCACTGCGGGGAAGGGGTGAAGACGGTGGTGAAC20.14174344436569808No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACACAGAAGGGTATCCGCCA20.14174344436569808No Hit
TGCCATCTTGCCACATCTACTTTTTCCCGCGTTTTCAAGGGTATCCGCCA20.14174344436569808No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTGTTTGGTTTCCCAGTCCACTA20.14174344436569808No Hit
TGCCGTCTAACCACATCTTGTGCCAATGTCGGAAGGGTATCCGCCAACTT20.14174344436569808No Hit
TGCCATCTTGCCACATCTGTGAAGATGGAAGGGTATCCGCCAACTTGAGG20.14174344436569808No Hit
GCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT20.14174344436569808No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAGTACATGGGAAGC20.14174344436569808No Hit
TGCCATCTTGCCACATCTACTCTGCGTTGATACCACTGCTTCCCATGACC20.14174344436569808Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTAGGAAGGGTATCCGCAACTTGAGGAGACCAAG20.14174344436569808No Hit
TGCCATCTTGCCACATCTTCCGCGCCCCGCCAAGGGTATCCGCCAACTTG20.14174344436569808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)