FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13000
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA660950.838461538461544No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA268720.66923076923077No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT12509.615384615384617No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1831.4076923076923078No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1721.323076923076923No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1090.8384615384615385No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC550.4230769230769231No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCACAGTCCACTA350.2692307692307692No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT320.24615384615384617No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT300.23076923076923078No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.21538461538461537No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT270.20769230769230768No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.19230769230769232No Hit
TGCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT190.14615384615384616No Hit
TGCCATCTTGCCACATCTTGTGCCAAGGGTATCCGCCAACTTGAGGAGAC190.14615384615384616No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT180.13846153846153844No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT160.12307692307692308No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA160.12307692307692308No Hit
TGCCATCTTGCCACATCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCC160.12307692307692308No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA160.12307692307692308No Hit
TGCCATCTTGCCACATCTTGTCATGTATGCTTCTTCAATCATATCAAAGC150.11538461538461539No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC150.11538461538461539No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA150.11538461538461539No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG140.10769230769230768No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGATATTTGGTTTCCCAGTCCACTA140.10769230769230768No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)