FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695
Total Bases245.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA31718.702064896755164No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA18610.973451327433628No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1156.784660766961652No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG895.2507374631268435No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG774.542772861356932No Hit
TGCCATCTTGCCACATCTTTCTCCAAGCAGTGGTATCAACGCAGAGTACA422.47787610619469Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTCGCCACTCCGGAAGGGTATCCGCCAACTTGA251.4749262536873156No Hit
TGCCATCTTGCCACATCTCCGCCACTCCGGAAGGGTATCCGCCAACTTGA201.1799410029498525No Hit
TGCCATCTTGCCACATCTTCTAGGACAAAGCAGTGGTATCAACGCAGAGT181.0619469026548671Clontech Universal Primer Mix Long (100% over 23bp)
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC160.943952802359882No Hit
TGCCATCTTGCCACATCTTGTGAAAGACAGTGTCGCCGAAAGTCCAAGGG130.7669616519174041No Hit
TGCCATCTTGCCACATCTTAGATCAGCAGCACACACGAAGCACTCCTCTC110.6489675516224189No Hit
TGCCATCTTGCCACATCTTGCCTTCCCAAGGGTATCCGCCAACTTGAGGA80.471976401179941No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG80.471976401179941No Hit
TGCCATCTTGCCACATCTTTTCCCCTGCCCCTGATCCCTGGCTCCCTGCC70.41297935103244837No Hit
TGCCATCTTGCCACATCTTAGGAGAGGAGGAAGGAAGGGTATCCGCCAAC70.41297935103244837No Hit
TGCCATCTTGCCACATCTCTCAGGACCGACTGACCCAAGGGTATCCGCCA70.41297935103244837No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG70.41297935103244837No Hit
TGCCATCTTGCCACATCTGCGGAAGGGTATCCGCCAACTTGAGGAGACCA60.35398230088495575No Hit
TGCCATCTTGCCACATCTCATCTTCTTTCTCAAGGGTATCCGCCAACTTG60.35398230088495575No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC60.35398230088495575No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA50.2949852507374631No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA50.2949852507374631No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT50.2949852507374631No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT50.2949852507374631No Hit
TGCCATCTTGCCACATCTGACGGGAAGGGTATCCGCCAACTTGAGGAGAC50.2949852507374631No Hit
TGCCATCTTGCCACATCTGATCTTGTTCTATCGGGTTTAAAGCAGTGGTA50.2949852507374631No Hit
TGCCATCTTGCCACATCTTACGGGAAGGGTATCCGCCAACTTGAGGAGAC50.2949852507374631No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.2359882005899705No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.2359882005899705No Hit
TGCCATCTTGCCACATCTTCTCCTTCTTCAAGGGTATCCGCCAACTTGAG40.2359882005899705No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG40.2359882005899705Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCTTCTCCTCTCTTCCAAGGGTATCCGCCAACA40.2359882005899705No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.2359882005899705No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT40.2359882005899705No Hit
TGCCATCTTGCCACATCTTGCGGCACATGGACCTGAACCATGCGCGTCTA40.2359882005899705No Hit
TGCCATCTTGCCACATCTTTACAACTCTAGAAAGAGAAGAAAACACAAAC40.2359882005899705No Hit
TGCCATCTTGCCACATCTGTCTGTTGAAGATGGAAGGGTATCCGCCAACT40.2359882005899705No Hit
TGCCATCTTGCCACATCTACCTTCCTCAAGGGTATCCGCCAACTTGAGGA40.2359882005899705No Hit
TGCCATCTTGCCACATCTTGGTGCCCTCAAGCAGTGGTATCAACGCAGAG40.2359882005899705Clontech Universal Primer Mix Long (100% over 23bp)
TGCCATCTTGCCACATCTTCCCAATCCTGATGGAAGGGTATCCGCCAACT40.2359882005899705No Hit
TGCCATCTTGCCACATCTTAGCCTTCCCAAGGGTATCCGCCAACTTGAGG40.2359882005899705No Hit
TGCCATCTTGCCACATCTTTCTCAAAGCAGTGGTATCAACGCAGAGTACA40.2359882005899705Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCGTCTAACCACATCTTTCTCCAAGCAGTGGTATCAACGCAGAGTACA30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTTCCATCAGAACCAAACCTCCTGCAGCGAAAG30.17699115044247787No Hit
TGCCATCTTGCCACATCTTATCTTGTTCTATCGGGTTTAAAGCAGTGGTA30.17699115044247787No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT30.17699115044247787No Hit
TGCCATCTTGCCACATCTGTTCTGTTGAAGATGGAAGGGTATCCGCCAAC30.17699115044247787No Hit
TGCCATCTTGCCACATCTGGCTAATTTTTGTATTTTCAGTAGGGTATCCG30.17699115044247787No Hit
TGCCATCTTGCCACATCTTGCTGATCGCCATTTCCGACGCGAAGGGCACG30.17699115044247787No Hit
TGCCATCTTGCCACATCTTGGGGAGAGTTCTCTTTTCTTTGGGAAGGGTA30.17699115044247787No Hit
TGCCATCTTGCCACATCTTCTTGGCAAAGCAGTGGTATCAACGCAGAGTA30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCATCTTGCCACATCTCCGCCACTCCGGAAGGGTATCCGCCAACACAG30.17699115044247787No Hit
TGCCATCTTGCCACATCTAATTCCCTCCGCCTTTCCCCGCCCCGCCTCAA30.17699115044247787No Hit
TGCCATCTTGCCACATCTCCGCCACTCTGGAAGGGTATCCGCCAACTTGA30.17699115044247787No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA30.17699115044247787Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCCCGCGCCCCGCCAAGGGTATCCGCCAACTTG30.17699115044247787No Hit
TGCCATCTTGCCACATCTTAGCCTCCGAAGCAGTGGTATCAACGCAGAGT30.17699115044247787Clontech Universal Primer Mix Long (95% over 24bp)
TGCCATCTTGCCACATCTTCGCCACTCCGGAAGGGTATCCGCCAACACAG30.17699115044247787No Hit
TGCCATCTTGCCACATCTCTCGGCCGATCGGAAGGGTATCCGCCAACTTG30.17699115044247787No Hit
TGCCATCTTGCCACATCTCGGGGAGAGTTCTCTTTTCTTTGGGAAGGGTA30.17699115044247787No Hit
TGCCATCTTGCCACATCTTTCTCTAAGCAGTGGTATCAACGCAGAGTACA30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTAAGCAGTGGTATCAACGCAGAGTACATGG30.17699115044247787Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGGGAGAGGAGGAAGGAAGGGTATCCGCCAAC30.17699115044247787No Hit
TGCCATCTTGCCACATCTGACGGGAAGGGTATCCGCCAACACAGAAGGGT30.17699115044247787No Hit
GAGGACATCTTATGGTGCACTGGGGCAAACTGAGGTATTGAGGAGACCAA30.17699115044247787No Hit
TGCCATCTTGCCACATCTTAGAACAGGCTGTAAGAGCACCTCAGGCAGCC30.17699115044247787No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT30.17699115044247787No Hit
AAGGTGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGATG20.11799410029498525No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCTAGGACGAAGCAGTGGTATCAACGCAGAGT20.11799410029498525Clontech Universal Primer Mix Long (100% over 23bp)
TGCCATCTTGCCACATCTTCATCCTCTCCCATGTCGTCATCATCATCCTC20.11799410029498525No Hit
TGCCGTCTAACCACATCTCCGCCACTCCGGAAGGGTATCCGCCAACTTGA20.11799410029498525No Hit
TGCCATCTTGCCACATCTCCACCCGAAGGGTATCCGCCAACTTGAGGAGA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTTAAGAATGCCAAAAATAACTAAAATACTGTC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACTTGAGGAGACCAAGTCTC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA20.11799410029498525No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCGCCAACTTGAGG20.11799410029498525No Hit
TGCCATCTTGCCACATCTCCTGGGAACTGCCTCACCCTCAGCACCAGCCC20.11799410029498525No Hit
TGCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT20.11799410029498525No Hit
TGCCATCTTGCCACATCTCATCTGCAGTCACTGTAACGAAACAGGGTTTC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCCATTTCCTAAGCAGTGGTATCAACGCAGAG20.11799410029498525Clontech Universal Primer Mix Long (100% over 22bp)
GAGGACATCTTATGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACG20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGCAAGGGTATCCGCCAACTTGAGGAGACCAA20.11799410029498525No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT20.11799410029498525No Hit
TGCCATCTTGCCACATCTGCGCACTTCACACCACACGGAAAGCAGTGGTA20.11799410029498525No Hit
TGCCATCTTGCCACATCTCAAGCCAAGCAGTGGTATCAACGCAGAGTACA20.11799410029498525Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTCCGCCACTATGGAAGGGTATCCGCCAACACAG20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCAGCTCCATGCACTCCTGCACCGTCGGAAGG20.11799410029498525No Hit
TGCCGTCTAACCACATCTTCTAGGACAAAGCAGTGGTATCAACGCAGAGT20.11799410029498525Clontech Universal Primer Mix Long (95% over 24bp)
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGGAGAGGAGGATGGAAGGGTATCCGCCAAC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTATCTGGAAGGGTATCCGCCAACTTGAGGAGA20.11799410029498525No Hit
TGCCGTCTAACCACATCTCTCAGGACCGACTGACCCAAGGGTATCCGCCA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC20.11799410029498525No Hit
TGCCATCTTGCCACATCTATGGAAGGGTATCCGCCAACTTGAGGAGACCA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGGAAGCAGTGGTATCAACGCAGAGTACATG20.11799410029498525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA20.11799410029498525Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTCCGCGCCCCGCCCCCGGAGCCCCGCGGACGC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCTTCCTCTTCCTTCAAGGGTATCCGCCAACT20.11799410029498525No Hit
GAGGACATCTTGCCACATCTCCGCCACTCCGGAAGGGTATCCGCCAACTT20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGGAAGGGTATCCGCCAACTTGAGGAGACCA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGCAGGGGTATCCGCCAACACAGAAGGGTAT20.11799410029498525No Hit
TGCCATCTTGCCACATCTGTGAAGATGGAAGGGTATCCGCCAACTTGAGG20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAAGCCAAGCAGTGGTATCAACGCAGAGTACA20.11799410029498525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCCCCACAAGCAGTGGTATCAACGCAGAGT20.11799410029498525Clontech Universal Primer Mix Long (100% over 24bp)
GAGGACATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTG20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCTTCATCAAACTCCTCTTCTTCACCATCCTC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTATGTCTCTTCACCGTAAGGGTATCCGCCAAC20.11799410029498525No Hit
TGCCATCTTGCCACATCAGGGTATCCGCCAACACAGAAGGGTATCCGCCA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACACAGAGTACATGGAAAGC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGTGCCCTTAAGCAGTGGTATCAACGCAGAG20.11799410029498525Clontech Universal Primer Mix Long (95% over 23bp)
AAGGTGGATGGTGAGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGTGCATCTTGCCACATCTAGGGTATCCGCCA20.11799410029498525No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGCCACTCCGGAAGGGTATCCGCCAACTTGA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGTCTCAGGAAGCAGTGGTATCAACGCAGAGT20.11799410029498525Clontech Universal Primer Mix Long (100% over 23bp)
TGCCATCTTGCCACATCTTTTCCGAAGGGTATCCGCCAACTTGAGGAGAC20.11799410029498525No Hit
TGCCATCTTGCCACATCTCTGGAAGGGTATCCGCCAACTTGAGGAGACCA20.11799410029498525No Hit
GAGGACATCTTATGGGGGCAAACTGAGGATTGAGGAGACCAAGTCTCTGC20.11799410029498525No Hit
AAGGTGGATGGTGATGTGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAAGCTAAGCAGTGGTATCAACGCAGAGTACA20.11799410029498525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCATCTTGCCACATCTGCGGAAGGGTATCCGCCAACTTGAGGAGACCAA20.11799410029498525No Hit
TGCCATCTTGCCACATCTGGAAGGGTATCCGCCAACTTGAGGAGACCAAG20.11799410029498525No Hit
GCCATCTTGCCACATCTTTCTCCAAGCAGTGGTATCAACGCAGAGTACAT20.11799410029498525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGTCTTGGCGCTGCTTCTCAGGATCTCCACAG20.11799410029498525No Hit
TGCCATCTTGCCACATCTAGGGAGAGGAGGAAGGAAGGGTATCCGCCAAC20.11799410029498525No Hit
TGCCATCTTGCCACATCTTAGCCACTCTGGAAGGGTATCCGCCAACTTGA20.11799410029498525No Hit
GAGGACATCTTATGGTGGCAAACTGAGTATTGAGGAGACCAAGTCTCTGC20.11799410029498525No Hit
GAGGACATCTTATGGTGCACGGGGGCAAACTGAGGTATTGAGGAGACCAA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGATTTGTGGAAGGGTATCCGCCAACTTGAGG20.11799410029498525No Hit
TGCCATCTTGCCACATCTTGGGGAGAGTTCTCTTTTCTTTGTGAAGGGTA20.11799410029498525No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT20.11799410029498525No Hit
AAGGTGGATGGTGATGTGGTCTCCAATTTAGCCACCTGCTTCCGGGCTGA20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCGGAAGGGTATCCGCCAACTTGAGGAGACCA20.11799410029498525No Hit
TGCCGTCTAACCACATCTTCAGCTCCATGCACTCCTGCACCGTCGGAAGG20.11799410029498525No Hit
TGCCATCTTGCCACATCTTCTCCTTCTTCAAGGGTATCCGCCAACACAGA20.11799410029498525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)