FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4976
Total Bases721.1 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC280056.27009646302251No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC64713.0024115755627No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1583.1752411575562705No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1362.7331189710610935No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1152.3110932475884245No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT420.8440514469453377No Hit
GTTGGCGGATACCCTTCGCATAAGGGCTTCTGACGGATGCCTTGGCATTG300.6028938906752411No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT190.38183279742765275No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA180.3617363344051447No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT140.28135048231511256No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG140.28135048231511256No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT130.2612540192926045No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA120.2411575562700965No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA110.22106109324758844No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC110.22106109324758844No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG110.22106109324758844No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.22106109324758844No Hit
TACCTCAGTTTGCCCCCATGGTGAAATGGAACAAGAAACAGATGGGTAGA100.20096463022508038No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT90.18086816720257234No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACATACACGCAAAATAC90.18086816720257234No Hit
TACCTCAGTTTGCCCCAGTGCACCATAAGATGTCCTCAACTGGTGTAGAA90.18086816720257234No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT90.18086816720257234No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC80.1607717041800643No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.14067524115755628No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACGCACGCAAAATAC70.14067524115755628No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC70.14067524115755628No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT70.14067524115755628No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC70.14067524115755628No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC70.14067524115755628No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA60.12057877813504825No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC60.12057877813504825No Hit
GTTGGCGGATACCCTTGGAATAGAAAGATGTGGCAAGATGGCAAACTGGT60.12057877813504825No Hit
GTTGGCGGATACCCTTCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTT60.12057877813504825No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC60.12057877813504825No Hit
TACCTCAGTTTGCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGAT60.12057877813504825No Hit
TACCTCAGTTTGCCCCCGTGCACCATAAGATGTCCTCAACTGGTGTAGAA60.12057877813504825No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGATGGCGGAT50.10048231511254019No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT50.10048231511254019No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACGCGCAAAATAC50.10048231511254019No Hit
GTGTCGCTACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT50.10048231511254019No Hit
CGAAGTAGACCACGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.10048231511254019No Hit
TACCTCAGTTTGCCCCCATGGAGCCGACAGGGGAATAAGCCAATGGGTTG50.10048231511254019No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAA50.10048231511254019No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT50.10048231511254019No Hit
GTTGGCGGATACCCTTCTCCCCCCGCCGCCGCCCCCACGCGGCGCTCCCC50.10048231511254019No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC50.10048231511254019No Hit
TACCTCAGTTTGCCCCCCCCTTTTTGGGCGTCCTAGTAAGATGTGGCAAG50.10048231511254019No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTGCACACACGCAAAATAC50.10048231511254019No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.10048231511254019No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATAC50.10048231511254019No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)