FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964
Total Bases284.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC94047.86150712830957No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1758.910386965376782No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG763.8696537678207736No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT753.818737270875764No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG613.105906313645621No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG391.985743380855397No Hit
TGCCATCTTGCCACATCTATATCAACCAACACCTTTTCTGAAGGGTATCC251.2729124236252547No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC170.8655804480651732No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT140.7128309572301426No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT130.6619144602851323No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA110.560081466395112No Hit
TGCCATCTTGCCACATCTTGTGTTAAGCAGTGGTATCAACGCAGAGTACA100.5091649694501018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTATATCAACCAACACCTTTTCGGAAGGGTATCC90.45824847250509165No Hit
TGCCATCTTGCCACATCTGACTTAACAAACCGCCTGCGGGAAGGGTATCC70.3564154786150713No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG70.3564154786150713No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT70.3564154786150713No Hit
TGCCATCTTGCCACATCTTTCTTAGTGTAAAAGCCCAGTGGTGTCACCAG60.30549898167006106No Hit
TGCCATCTTGCCACATCTTCTTCTTAATGACTAATGAGACACAGGCACTC60.30549898167006106No Hit
TGCCATCTTGCCACATCTCAAGGGTATCCGCCAACTTGAGGAGACCAAGT60.30549898167006106No Hit
GAGGACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCTTGTGGAT60.30549898167006106No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA60.30549898167006106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT50.2545824847250509No Hit
TGCCATCTTGCCACATCTTAGACCCCCGGATTTGCCGAAGGGTATCCGCC50.2545824847250509No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT50.2545824847250509No Hit
TGCCGTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC50.2545824847250509No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG50.2545824847250509Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCAATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT50.2545824847250509No Hit
TCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT40.20366598778004072No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG40.20366598778004072No Hit
TGCCATCTTGCCACATCTTGCAAGGGTATCCGCCAACTTGAGGAGACCAA40.20366598778004072No Hit
TGCCATCTTGCCACATCTAGCAAATATTCAAACGAGAACTTTGAAGGCCG40.20366598778004072No Hit
TGCCATCTTGCCACATCTTGAGATTGTTTTAGAAAACCTGAGTTTTCATA40.20366598778004072No Hit
TGCCATCTTGCCACATATTAGAAGTTTAGTTGAAATCATTGAACATGGTC30.15274949083503053No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA30.15274949083503053No Hit
TGCCATCTTGCCACATCTTGGAAGTTTAGTTGAAATCATTGAACATGGTC30.15274949083503053No Hit
TGCCATCTTGCCACATCTGGGTATCCGCCAACTTGAGGAGACCAAGTCTC30.15274949083503053No Hit
TGCCATCTTGCCGCATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC30.15274949083503053No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCCAAGGGTATCCGCCAACTTG30.15274949083503053No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT30.15274949083503053No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT30.15274949083503053No Hit
TGCCATCTTGCCACATCTTTGTCATCTGTCATTCTTCCAGGAGTTAAAGC30.15274949083503053No Hit
TGCCATCTTGCCACATCTCTTGAAAATCCGGGGGAAGGGTATCCGCCAAC30.15274949083503053No Hit
TGCCATCTTGCCACATCTGTTCTGTTGAAGATGGAAGGGTATCCGCCAAC30.15274949083503053No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG30.15274949083503053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCCATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT30.15274949083503053No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGATC30.15274949083503053No Hit
GAGGACATCTTATGGTGCACTGGGGCAAACTGAGGTATTGAGGAGACCAA30.15274949083503053No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC30.15274949083503053No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC30.15274949083503053No Hit
TGCCATCTTGCCACATCTTACTTAACAAACCGCCTGCGGGAAGGGTATCC30.15274949083503053No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAGCATGGTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGACTCAGCAAGGGT20.10183299389002036No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT20.10183299389002036No Hit
TGCCATCTTGCCACATCTTGCCTTCCCAAGGGTATCCGCCAACTTGAGGA20.10183299389002036No Hit
TGCCATCTTGCCACATCTTACCATCCTGGGCCTGGCAGTGCTACGGGCAC20.10183299389002036No Hit
GAGGACATCTTGCCACATCTCCTTTAAGTTTCAGCTTTGCAACCATACTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTAGAAGCTTAGTTGAAATCATTGAACATGGTC20.10183299389002036No Hit
TGCCACTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT20.10183299389002036No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA20.10183299389002036No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGGACATGGTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACGTGGTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTCTCAGTGAGCCAAGGATTGCTGATCTCTGCT20.10183299389002036No Hit
GAGGACATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGG20.10183299389002036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCCCGCGCCCCGCCAAGGGTATCCGCCAACTTG20.10183299389002036No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCAAGGGTATCCGCCAACTTGA20.10183299389002036No Hit
TGCCATCTTGCCACATCTCCTTTAAGTTTCAGCTTTGCAACCATACTCCC20.10183299389002036No Hit
AGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCGTTGAACATGGTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA20.10183299389002036No Hit
GAGGACATCTTGCCACATCTATATCAACCAACACCTTTTCGGAAGGGTAT20.10183299389002036No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGAAGGGTATCCGCCAA20.10183299389002036No Hit
TGCCATCTTGCCACATCTAAAGCAGTGGTATCAACGCAGAGTACATGGGT20.10183299389002036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTTATCAACGCAGAGTACATGGGAAGCAGTGGT20.10183299389002036No Hit
TGCCATCTTGCCACATCTTCTCTAATTTGCCAAGTACTCTTCAGTCAGGC20.10183299389002036No Hit
TGCCATCTTCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT20.10183299389002036No Hit
TGCCATCTTGCCACATCTTCCAAGCAGTGGTATCAACGCAGAGTACATGG20.10183299389002036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT20.10183299389002036No Hit
TGCCATCTTGCCACATCTGTGAAGATGGAAGGGTATCCGCCAACTTGAGG20.10183299389002036No Hit
TGCCGTCTAACCACATCTATATCAACCAACACCTTTTCGGAAGGGTATCC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTAGGCTCCCCCAAAGCAGTGGTATCAACGCAG20.10183299389002036Clontech Universal Primer Mix Long (95% over 21bp)
TGCCATCTTGCCACATCTTAGACCCCCGGATTTGCCTAAGGGTATCCGCC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTCTTTAAGTTTCAGCTTTGCAACCATACTCCC20.10183299389002036No Hit
TGCCATCTTGCCACATCTTCTTACAGGAAGGGGAAAAAACAGTTCTGTTC20.10183299389002036No Hit
TGCCATCTTGCCACATCTCATTTCTGTCAATTTCATATCTGATTTTTTTT20.10183299389002036No Hit
TGCCATCTTGCCACTCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT20.10183299389002036No Hit
GAGGACATCTTATGGTGCACTGAGCTGGGAGTGTATGAGGTCTGTTTGAG20.10183299389002036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)