FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17632
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1113263.135208711433755No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC12487.078039927404718No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC3001.7014519056261341No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2701.531306715063521No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2691.5256352087114338No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2491.4122050816696916No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1931.094600725952813No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1831.037885662431942No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1260.7146098003629764No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1250.7089382940108893No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1220.6919237749546279No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA980.5558076225045372No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.3856624319419238No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT660.37431941923774953No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA640.3629764065335753No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT550.31193284936479126No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG400.2268602540834846No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA340.1928312159709619No Hit
GTTGGCGGATACCCTTCACCCTCCTCCTACCACCAAGCTCTCCGGCAGTC320.18148820326678766No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG320.18148820326678766No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA300.17014519056261343No Hit
GTTGGCGGATACCCTAACGGAGGCGTACGAAGATGTGGCAAGATGGCAAA300.17014519056261343No Hit
GTTGGCGGATACCTTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG280.1588021778584392No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG270.1531306715063521No Hit
GTTGGCGGATACCCTTCCCCCTCCTCCTACCACCAAGCTCTCCGGCAGTC260.147459165154265No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG240.13611615245009073No Hit
GTTGGCGGATACACTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG230.13044464609800363No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCGAAAGATTCG220.12477313974591651No Hit
GTTGGCGGATACCCTTGGCTTCCTCCCCTAGCCCTCCTCCTCCTTCCTCT220.12477313974591651No Hit
GTTGGCGGATATCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG220.12477313974591651No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA210.1191016333938294No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC200.1134301270417423No Hit
GTTGGCGGATACCCTTCCCCTCCTCCTACCACCAAGCTCTCCGGCAGTCA190.10775862068965517No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAACCAAAAGATTCG190.10775862068965517No Hit
GTTGGCGGATACCCTTGGGTCATTTCAAAGAGGGCTTATGAGGCTGTGAA190.10775862068965517No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACGGAATCAAAAGATTCG180.10208711433756805No Hit
TACCTCAGTTTGCCCCCATGCTTCAGGCCATCCCTGGCTTGAAGGTGGGG180.10208711433756805No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)