FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5687
Total Bases824.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT387868.19061016353086No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC3245.697204149815368No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2324.0794795146826095No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1983.481624758220503No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA530.931950061543872No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA520.9143660981185159No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG140.24617548795498506No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT130.22859152452962897No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC130.22859152452962897No Hit
TGCCATCTTGCCACATCTTGTCTGAAGGGTATCCGCCAACTTGAGGAGAC130.22859152452962897No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT120.21100756110427288No Hit
TGCCATCTTGCCACATCTATGCTGAAACTTAGATTTGTTTTTGGAAGGGT120.21100756110427288No Hit
TGCCATCTTGCCACATCTTGTCGGAAGGGTATCCGCCAACTTGAGGAGAC120.21100756110427288No Hit
TGCCATCTTGCCACATCTGAAGGCAGGAAGGGTATCCGCCAACTTGAGGA110.19342359767891684No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT100.17583963425356075No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG100.17583963425356075No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT100.17583963425356075No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG100.17583963425356075No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT100.17583963425356075No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT100.17583963425356075No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT90.15825567082820466No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT90.15825567082820466No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC80.1406717074028486No Hit
GAGGACATCTTATGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC80.1406717074028486No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC80.1406717074028486No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT70.12308774397749253No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGGAAGGGT70.12308774397749253No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC70.12308774397749253No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGATATTTGGTTTCCCAGT70.12308774397749253No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT60.10550378055213644No Hit
GAGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC60.10550378055213644No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA60.10550378055213644No Hit
TGCCATCTTGCCACATCTCGGGCGATGGCCTCCGTTGCCCTCGGCCGATC60.10550378055213644No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT60.10550378055213644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)