FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13440
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT963271.66666666666667No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC141510.52827380952381No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT2702.0089285714285716No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2631.9568452380952381No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2471.837797619047619No Hit
TGCCATCTTGCCACATCTTTTGTTAAATGAGGTCATCTTTTTGTCTTACT540.40178571428571425No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA330.24553571428571427No Hit
TGCCATCTTGCCACATCTCCTTGCCAATGGTGTAGTGCCCTCGGGCATAG240.17857142857142858No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG240.17857142857142858No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC240.17857142857142858No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT230.17113095238095238No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT220.1636904761904762No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT190.14136904761904762No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT180.13392857142857142No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT180.13392857142857142No Hit
TGCCATCTTGCCACATCTAAATCAGTGTGTGGCAGTAGGAAGGGTATCCG180.13392857142857142No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAAT180.13392857142857142No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT170.12648809523809523No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC170.12648809523809523No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT170.12648809523809523No Hit
TGCCATCTTGCCACATCTTTCACTGTTTTTGTATCTCGCACATGTATGTT150.11160714285714285No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC150.11160714285714285No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT140.10416666666666667No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT140.10416666666666667No Hit
GAGGACATCTTATGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC140.10416666666666667No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)