FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20163
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1006049.89336904230521No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA233811.59549670187968No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA227311.273124039081486No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT6583.263403263403263No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5142.5492238258195705No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5052.5045876109705896No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC3881.9243168179338392No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1640.8133710261369836No Hit
TGCCATCTTGCCACATCTTGCCAAGGATAAGCACCCACAGGTGCTTATTT860.4265238307791499No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC760.3769280365025046No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC620.30749392451520113No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT420.20830233596191042No Hit
TGCCATCTTGCCACATCTAACTGGCGCGAGGAAGGGTATCCGCCAACTTG380.1884640182512523No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC320.15870654168526507No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA310.15374696225760057No Hit
TGCCATCTTGCCACATCTCAAGCCAAGCAGTGGTATCAACGCAGAGTACA300.148787382829936Clontech Universal Primer Mix Long (96% over 26bp)
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT290.1438278034022715No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.1289490651192779No Hit
TGCCATCTTGCCACATCTTGATATTATCATTTTATCCACAGGGTTTGTCA250.12398948569161336No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT240.11902990626394881No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT240.11902990626394881No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG230.11407032683628429Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT220.10911074740861974No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG220.10911074740861974No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT210.10415116798095521No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG210.10415116798095521Clontech Universal Primer Mix Long (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)