FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32384
Total Bases4.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1485745.87759387351779No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA563117.388216403162055No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC32109.912302371541502No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA31669.776432806324111No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3901.204298418972332No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3120.9634387351778656No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1710.5280385375494071No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC940.29026679841897235No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT770.2377717391304348No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT650.20071640316205536No Hit
TGCCATCTTGCCACATCTTCCACAATTCGAAGCAGTGGTATCAACGCAGA630.1945405138339921Clontech Universal Primer Mix Long (95% over 22bp)
TGCCATCTTGCCACATCTTCGAAAGCCACCAAGTCCATTTCCTGGCTTTC630.1945405138339921No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG600.18527667984189722No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT500.15439723320158102No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT460.14204545454545456No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT460.14204545454545456No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG450.1389575098814229No Hit
TGCCATCTTGCCACATCTTTCTCTCAGCCGCCAAAGACCCCGTCCGGTAG440.1358695652173913No Hit
TGCCATCTTGCCACATCTTCACATTTGTAAGGTTTCTCTCCAGTATGAAT430.13278162055335968No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT420.12969367588932806No Hit
TGCCATCTTGCCACATCTGAAAAGTATAGGGTAGGCTGACCACAAAAGAT400.12351778656126483No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT400.12351778656126483No Hit
TGCCATCTTGCCACATCTCCCACAATTCGAAGCAGTGGTATCAACGCAGA400.12351778656126483Clontech Universal Primer Mix Long (100% over 21bp)
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT400.12351778656126483No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT350.10807806324110672No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT340.1049901185770751No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)