FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8982
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT344438.34335337341349No Hit
TGCCATCTTGCCACATCTGGAAGCAGTGGTATCAACGCAGAGTACATGGG8509.46337118681808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA8499.452237808951235No Hit
TGCCATCTTGCCACATCTTGAAGCAGTGGTATCAACGCAGAGTACATGGG6517.247828991315965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC5996.668893342240036No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2222.4716098864395457No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1651.837007348029392No Hit
TGCCATCTTGCCACATCTGAAAGCAGTGGTATCAACGCAGAGTACATGGG1461.625473168559341Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1111.2358049432197729No Hit
GAGGACATCTTGCCACATCTGGAAGCAGTGGTATCAACGCAGAGTACATG520.5789356490759296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTGCCACATCTTGAAGCAGTGGTATCAACGCAGAGTACATG430.47873524827432645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT410.4564684925406368No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA310.3451347138721888No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG270.30060120240480964No Hit
TGCCATCTTGCCACATCTGGAAGCAGTGGTATCAACGCAGAGTACATGGA220.2449343130705856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCTGGGAAGGGTATCCGCCAACTTGAGGAGACC210.23380093520374082No Hit
TGCCATCTTGCCACATCTTGAAGCAGTGGTATCAACGCAGAGTACATGGA200.222667557336896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG200.222667557336896No Hit
TGCCATCTTGCCACATCTTAGCCAAGGGTATCCGCCAACTTGAGGAGACC180.2004008016032064No Hit
TGCCATCTTGCCACATCTTTCATCGCCTCTCTACGCCAAGGGTATCCGCC180.2004008016032064No Hit
TGCCATCTTGCCACATCTTCTAACACGACTTCGCGAAGGGTATCCGCCAA170.18926742373636163No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC160.1781340458695168No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT150.16700066800267202No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG150.16700066800267202No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA140.15586729013582723No Hit
GAGGACATCTTGCCACATCTGAAAGCAGTGGTATCAACGCAGAGTACATG140.15586729013582723Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT120.13360053440213762No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT120.13360053440213762No Hit
TGCCATCTTGCCACATCTTGTTAGGGGAGGCAGGGCATCTGGGGAAGGGT120.13360053440213762No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC110.1224671565352928No Hit
TGCCATCTTGCCACATCTGGAAGCAGTGGTATCAACGCAGAGTACAAGGG110.1224671565352928Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT100.111333778668448No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT100.111333778668448No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA90.1002004008016032No Hit
TGCCATCTTGCCACATCTTAAAGCAGTGGTATCAACGCAGAGTACATGGG90.1002004008016032Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGTTAGGGGAGGCAGGGCATCTGGTGAAGGGT90.1002004008016032No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT90.1002004008016032No Hit
TGCCGTCTAACCACATCTTGAAGCAGTGGTATCAACGCAGAGTACATGGG90.1002004008016032Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTACGTCCAAGGGTATCCGCCAACTTGAGGAGAC90.1002004008016032No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC90.1002004008016032No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT90.1002004008016032No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT90.1002004008016032No Hit
TGCCATCTTGCCACATCTTGGCCAAGGGTATCCGCCAACTTGAGGAGACC90.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)