FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8982
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA328236.539746158984634No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC7608.461367178802048No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG5926.590959697172122No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2142.3825428635047876No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1661.848140725896237No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1601.781340458695168No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1451.6143397906924963Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1051.169004676018704No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT820.9129369850812736No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA670.7459363170786016No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC660.7348029392117569Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCAGATGTG630.7014028056112225Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCAAGATGTG590.6568692941438432Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCAAACTGGT310.3451347138721888No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG310.3451347138721888No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG290.3228679581384992No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACCCCATGTACTCTGC280.31173458027165446Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT260.2894678245379648No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGATGGCGGAT250.27833444667112No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTAC220.2449343130705856Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG220.2449343130705856No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG210.23380093520374082No Hit
GTTGGCGGATACCCTTCCCAGAGATGTGGCAAGATGGCAAACTGGTGTAG210.23380093520374082No Hit
GTTGGCGGATACCCTTGGACATCATCCCATGTACTCTGCGTTGATACCAC210.23380093520374082Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTGCCTGTGTTGGCGGATACCCATGTACTCTGCGTT200.222667557336896No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA200.222667557336896No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT190.2115341794700512No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG190.2115341794700512No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA180.2004008016032064No Hit
GTTGGCGGATACCCTTGCTCCCATGTACTCTGCGTTGATACCACTGCTTC180.2004008016032064Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGGCTAAGATGTGGCAAGATGGCAAACTGGTGTAG180.2004008016032064No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG170.18926742373636163No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCCCATGTACTCTGCGTT170.18926742373636163No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG170.18926742373636163No Hit
GTTGGCGGATACCCTTGGCGTAGAGAGGCGATGAAAGATGTGGCAAGATG160.1781340458695168No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG160.1781340458695168No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG160.1781340458695168No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG150.16700066800267202No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC150.16700066800267202No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTGTACTCTGCGTTGA150.16700066800267202No Hit
GTTGGCGGATACCCTTCGCGAAGTCGTGTTAGAAGATGTGGCAAGATGGC150.16700066800267202No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTTCAGATGTG140.15586729013582723Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA140.15586729013582723No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA140.15586729013582723No Hit
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCAA140.15586729013582723No Hit
GTTGGCGGATACCCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT140.15586729013582723Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACCCATGTACTCTGCGTTG140.15586729013582723No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA130.1447339122689824No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCTTGTACTCTGCGT130.1447339122689824No Hit
GTTGGCGGATACCCTGCTTCCATGCCCATGTACTCTGCGTTGATACCACT120.13360053440213762Clontech SMART CDS Primer II A (95% over 22bp)
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCTGCTTCCCATGTA120.13360053440213762No Hit
GTTGGCGGATACCCTTGTGTTGGCGGATACCCCTGTGTTGGCGGATACCC120.13360053440213762No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT120.13360053440213762No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT120.13360053440213762No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.1224671565352928No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.1224671565352928No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT110.1224671565352928No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTGCTTCCCATGTACTC110.1224671565352928No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT100.111333778668448Clontech SMART CDS Primer II A (96% over 26bp)
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG100.111333778668448No Hit
GTTGGCGGATACCCTTCGGGGTGAAGGTCCGGGCCTAACGCTCGACCCCA100.111333778668448No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT100.111333778668448No Hit
GTTGGCGGATACCCTTCACCAGATGCCCTGCCTCCCCTAACAAGATGTGG90.1002004008016032No Hit
GTTGGCGGATACCCTTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC90.1002004008016032Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA90.1002004008016032No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.1002004008016032No Hit
GTTGGCGGATACCCTTGGACGTAGATGTGGCAAGATGGCAAACTGGTGTA90.1002004008016032No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTACTCCTTCA90.1002004008016032No Hit
GTTGGCGGATACCCTTGGCCAAGATGTGGCAAGATGGCAAACTGGTGTAG90.1002004008016032No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA90.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)