FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34314
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1775551.74272891531153No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA631518.403567057177828No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC353310.29608905985895No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT14914.34516523867809No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2690.783936585650172No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1940.5653669056361834No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1910.5566241184356239No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGACTGA1190.346797225622195No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1010.2943405024188378No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT950.2768549280177187No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT830.24188377921548057No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT660.19234131841230984No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT630.18359853121175032No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT630.18359853121175032No Hit
TGCCATCTTGCCACATCTTGTGCCATCTTGCCACATCAAGGGTATCCGCC630.18359853121175032No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC520.15154164480969867No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT510.14862738240951215No Hit
TGCCATCTTGCCACATCTACATGCCCCTCAGCGCTGGGCAAATGCTTTCT510.14862738240951215No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG490.14279885760913913No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT450.1311418080083931No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG440.12822754560820657No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT420.12239902080783352No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT410.11948475840764704No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC410.11948475840764704No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGCATTTGGTTTCCCAGT410.11948475840764704No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT380.11074197120708748No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT360.10491344640671448No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT360.10491344640671448No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT350.10199918400652795No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC350.10199918400652795No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT350.10199918400652795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)