FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34314
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length95-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1494543.55365157078743No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC482214.052573293699364No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG417812.17578830797925No Hit
CGAAGTAGACCAGCGGCATCTCAGCCCCACCCTGGACCTTAGCTCAGCAC3601.0491344640671447No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2400.699422976044763No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1750.5099959200326397No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA1630.4750247712304016No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1580.460453459229469No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1410.4109109984262983No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1130.329311651221076No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT1050.30599755201958384No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1010.2943405024188378No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1010.2943405024188378No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG970.2826834528180917No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG890.25936935361659963No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT880.25645509121641313No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG840.24479804161566704No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACCCGCAAAATACTCCTTCA770.22439820481436148No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA750.21856968001398844No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.20691263041324243No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG660.19234131841230984No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.17194148161100425No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCACAAGATGTGGCAAGATG540.1573701696100717No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.1544559072098852No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATACTCCTTCA520.15154164480969867No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG500.14571312000932563No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCATTCA480.1398845952089526No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.1311418080083931No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGAAAAATACTCCTTCA450.1311418080083931No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT440.12822754560820657No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCCGCAC440.12822754560820657No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA430.12531328320802004No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA420.12239902080783352No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG420.12239902080783352No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA420.12239902080783352No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA420.12239902080783352No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG410.11948475840764704No Hit
TACCTCAGTTTGCACCCATGTCCCTTACACACACGCAAAATACTCCTTCA410.11948475840764704No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCCC400.11657049600746051No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG400.11657049600746051No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.113656233607274No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA380.11074197120708748No Hit
GTTGGCGGATACCCTTCCTGCCACCACCTCCACCCTGCCCTGGCCCACTT370.10782770880690097No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCCCCCTGGACCTTAGCTCAGCAC370.10782770880690097No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT350.10199918400652795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)