FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12458
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT714757.368759030341955No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC135010.83641033873816No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA3722.9860330711189595Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1861.4930165355594798No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA1811.4528816824530424Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1721.3806389468614546No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA1611.2923422700272917No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1511.2120725638144165No Hit
TGCCATCTTGCCACATCTTCCTTAGCATTCTGTTCTTTGAAATGGAAGGG1010.8107240327500401No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA880.7063734146733023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA860.6903194734307273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCCTCTCCACCCCTGCCAGATCTCTCAACCCCA670.5378070316262642No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG580.46556429603467653No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG400.321078824851501No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGT380.305024883608926Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGAAG380.305024883608926Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTCTCTCCACCCCTGCCAGATCTCTCAACCCCA380.305024883608926No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTG280.22475517739605072Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGG270.2167282067747632Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT270.2167282067747632No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGCA260.20870123615347566Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAG250.20067426553218815Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT230.18462032428961309No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGCAAGGGTATCCGCCA220.17659335366832557No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC200.1605394124257505No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGA190.152512441804463Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT170.13645850056188794No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAT160.12843152994060042Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT160.12843152994060042No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT160.12843152994060042No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC160.12843152994060042No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG160.12843152994060042No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA150.12040455931931289No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGGAAGGGTATCCGCCA150.12040455931931289No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT150.12040455931931289No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAG140.11237758869802536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT130.10435061807673783No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC130.10435061807673783No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT130.10435061807673783No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)