FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22209
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT986744.42793462109955No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA520823.44995272186951No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC247911.162141474177135No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT9834.426133549461929No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4101.8460984285649962No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3041.3688144445945338No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG830.3737223648070602No Hit
TGCCATCTTGCCACATCTTCATCAGGGGGCATGTAAATGGAAGGGTATCC520.23413931289117024No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC480.21612859651492636No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT450.2026205592327435No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT370.16659912648025577No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA300.135080372821829No Hit
TGCCATCTTGCCACATCTTCCTCTTGGGAAGATCTTACTAACAAGCAGTG290.130577693727768No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT270.1215723355396461No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT260.11706965644558512No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG260.11706965644558512No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT260.11706965644558512No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG260.11706965644558512No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT240.10806429825746318No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT240.10806429825746318No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT230.10356161916340223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)