FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12878
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT762159.17844385774188No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA10828.401925764870322No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC7285.653051716104985No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5294.107780711290573No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3973.082776828700109No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCACGAGT800.6212144742972511No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT780.6056841124398199No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA660.5125019412952322No Hit
TGCCATCTTGCCACATCTACCCAGACTCCTCGCCGACGTGGCCTGGATGA590.45814567479422263No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG370.28731169436247866No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAAGGGTATCCGCCAACTTGAGG320.24848578971890045No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC320.24848578971890045No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT270.20965988507532224No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT250.19412952321789098No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT250.19412952321789098No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG250.19412952321789098No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT230.1785991613604597No Hit
TGCCATCTTGCCACATCTACCCAGACTCCTCGCCGACGTGGCCTGGAGGA220.17083398043174408No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCAAGGGTATCCGCCAACTTGA210.1630687995030284No Hit
TGCCATCTTGCCACATCTTGCCAAGGGTATCCGCCAACTTGAGGAGACCA180.1397732567168815No Hit
TGCCATCTTGCCACATCTACGCTACGCCGCGACGAGTAGGAGGGCCGCTG180.1397732567168815No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT170.13200807578816587No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTGTTTGGTTTCCCAGT170.13200807578816587No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG170.13200807578816587No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGATATTTGGTTTCCCAGT160.12424289485945023No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT160.12424289485945023No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT150.1164777139307346No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT150.1164777139307346No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA150.1164777139307346Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT140.10871253300201895No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC130.10094735207330331No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA130.10094735207330331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)