FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34378
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1423041.392751178079No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC485314.116586188841701No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC28118.176740938972598No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG28108.173832101925651No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC6511.893652917563558No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5441.5824073535400547No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1290.37523997905637324No Hit
GTTGGCGGATACCCTTGCTGATGTGGCCTACAGCTGCCTGGCTCATTACC1260.36651346791552736No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1160.3374250974460411No Hit
GTTGGCGGATACCCTTCCTGATGCGTTAGTTCCCAGGTGGAGCTGCATGT990.28797486764791436No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT970.2821571935540171No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG930.2705218453662226No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT910.2647041712723253No Hit
GTTGGCGGATACCCTTGGCAACTAGTGCATTTGGACTGGAACTACTTGGG900.2617953342253767No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA900.2617953342253767No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG850.24725114899063352No Hit
GTTGGCGGATACCCTTCATCCTAAAGTAAAATTGTAGTTTTATAGTAAGA780.22688928966199312No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT740.21525394147419863No Hit
GTTGGCGGATACCCTTGCAAGATGGCACAGATGTGGCAAGATGGCAAACT730.21234510442725No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGACGTGGCGACCCGCTGAA730.21234510442725No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA690.20070975623945547No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.19780091919250684No Hit
GTTGGCGGATACCCTTCTCCACAACAGCCCTCAAGGCCCTCTTTCGAGAG650.18907440805166095No Hit
GTTGGCGGATACCCTTGCTTGGAATAATAGCCTTTAATCATTATCCTCAG630.18325673395776368No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG630.18325673395776368No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.16871254872302055No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA570.1658037116760719No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.1541683634882774No Hit
GTTGGCGGATACCCTTCCTGATGTGGCCTACAGCTGCCTGGCTCATTACC510.14835068939438012No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG500.1454418523474315No Hit
GTTGGCGGATACCCTTCCCAATCATGGCAAGCCAACGCCACTTATCCAGT500.1454418523474315No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT490.14253301530048287No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG490.14253301530048287No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT490.14253301530048287No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.13962417825353424No Hit
GTTGGCGGATACCCTTCCTCCTAAAGTAAAATTGTAGTTTTATAGTAAGA450.13089766711268835No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT450.13089766711268835No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.12798883006573972No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC420.12217115597184244No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG400.1163534818779452No Hit
GTTGGCGGATACCCTTCCACTTTTCTGCCATGCTACGAAGCAGCACAAGG390.11344464483099656No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA390.11344464483099656No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA380.11053580778404794No Hit
GTTGGCGGATACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATA370.10762697073709931No Hit
GTTGGCGGATACCCTTGCTTGACTGCTGGTAAGCACTTGACATCAGTCTC360.10471813369015068No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA360.10471813369015068No Hit
GTTGGCGGATACCCTTCTCCACAACAGCCCTCAAGGCCCTCTTTCGAAAG350.10180929664320205No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG350.10180929664320205No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT350.10180929664320205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)