FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813
Total Bases262.7 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC60

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC29816.43684500827358No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC29116.050744622173195No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG583.1991174848317705No Hit
GTTGGCGGATACCCTTGTGGCAAGATGGCAGATGTGGCAAGATGGCAAAC412.2614451185879756No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT372.0408163265306123No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG331.8201875344732488No Hit
GTTGGCGGATACCCTTCCCGCAGGCGGTTTGTTAAGTCAGATGTGGCAAG281.5444015444015444No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT201.1031439602868174No Hit
TACCTCAGTTTGCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGAT191.0479867622724766No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT191.0479867622724766No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC180.9928295642581356No Hit
GTTGGCGGATACCCTTGCTGGAAAGCAAGATGTGGCAAGATGGCAAACTG180.9928295642581356No Hit
GTTGGCGGATACCCTTCACGCAGGCGGTTTGTTAAGTCAGATGTGGCAAG170.9376723662437948No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA130.7170435741864313No Hit
TACCTCAGTTTGCCCCCTTTCCCCTTTTTTGGCGTCCTAGTAAGATGTGG120.6618863761720905No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGT120.6618863761720905No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC110.6067291781577496Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCCATAACACACGGGATAGCCTTCTGGCGCAGTCC110.6067291781577496No Hit
GTTGGCGGATACCCAGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG100.5515719801434087No Hit
GTTGGCGGATACCCTTGGCACAAGATGTGGCAAGATGGCAAACTGGTGTA90.4964147821290678No Hit
GTTGGCGGATACCCTTCCCGCAGGCGGTTTGTTAAGTAAGATGTGGCAAG90.4964147821290678No Hit
GTTGGCGGATACCCTTGGGAAACCGGAGCTAATACCGGATAAACCCTTTC80.441257584114727No Hit
GTTGGCGGATACCCTTCACGCAGGCGGTTTGTTAAGTAAGATGTGGCAAG80.441257584114727No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA80.441257584114727No Hit
GTTGGCGGATACCCTGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG70.3861003861003861No Hit
GTTGGCGGATACCCTTGCTGGAAAGCGAGATGTGGCAAGATGGCAAACTG70.3861003861003861No Hit
GTTGGCGGATACCCTTGGCAAGATGGCACAAGATGTGGCAAGATGGCAAA70.3861003861003861No Hit
GTTGGCGGATACCAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG70.3861003861003861No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG70.3861003861003861No Hit
GTTGGCGGATACCCTTGTGGCAAGATGGAAGATGTGGCAAGATGGCAAAC60.3309431880860452No Hit
GTTGGCGGATACCCTTGCAAGATGTGGCAAGATGGCAAACTGGTGTAGAA60.3309431880860452No Hit
TACTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG60.3309431880860452No Hit
TACCTCAGTTTGCCCCCATGCAAACTCCGAATACCAGTAAGTACTATCCG60.3309431880860452No Hit
TACCTCAGTTTGCCCCCGTGCACCATAAGATGTCCTCAACTGGTGTAGAA60.3309431880860452No Hit
GTTGGCGGATACCCTAGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT60.3309431880860452No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG60.3309431880860452No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA60.3309431880860452No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT60.3309431880860452No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGACAGATGTGGCAAGATGGCAA50.27578599007170435No Hit
GTTGGCGGATACCCTTGCTGGTACAGATGTGGCAAGATGGCAAACTGGTG50.27578599007170435No Hit
GTTGGCGGATACCCTTGCAAGATGGCACAGATGTGGCAAGATGGCAAACT50.27578599007170435No Hit
GTTGGCGGATACCCTTCGGTTTGTTAAGTCAGATGTGGCAAGATGGCAAA50.27578599007170435No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACCCCATGTACTCTGC50.27578599007170435Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTGCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA50.27578599007170435No Hit
GTTGGCGGATACCCAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT50.27578599007170435No Hit
GTTGGCGGATACCCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCA40.2206287920573635No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG40.2206287920573635No Hit
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCAA40.2206287920573635No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCAAGATGTGGCAAGATGG40.2206287920573635No Hit
TACCTCAGTTTGCCCCAGTGCACCATAAGATGTCCTCAACTGGTGTAGAA40.2206287920573635No Hit
GTTGGCGGATACCCTTCCAGATGTGGCAAGATGGCAAACTGGTGTAGAAC40.2206287920573635No Hit
GTTGGCGGATACCCTTCCTGGAAAGCAAGATGTGGCAAGATGGCAAACTG40.2206287920573635No Hit
TACCTAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG40.2206287920573635No Hit
GTTGGCGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTGG40.2206287920573635No Hit
GTTGGGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTGG30.1654715940430226No Hit
GTTGGCGGATACCCTTGGCAAGATGTGGCAAGATGGCAAACTGGTGTAGA30.1654715940430226No Hit
GTTGGCGGATACCCTTGCTATTTCTAGCTCTAAAACCCGGCCAGAGCAGT30.1654715940430226No Hit
GTTGGCGGATACCCTTCAAGATGTGGCAAGATGGCAAACTGGTGTAGAAC30.1654715940430226No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGATGGCGGAT30.1654715940430226No Hit
GTTGGCGGATACCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT30.1654715940430226No Hit
TACCTCAGTTTGCCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGT30.1654715940430226No Hit
GTTGGCGGATACCCTTCCATCTGACTTCCTACTCCTAAGATGTGGCAAGA30.1654715940430226No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACTAAGATGTGGCAAGATGG30.1654715940430226No Hit
GTTGGCGGATACCCCAGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT30.1654715940430226No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACCCCATGTACTCTGC30.1654715940430226Clontech SMART CDS Primer II A (95% over 21bp)
GTTGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAAC30.1654715940430226No Hit
GTTGGCGGATACCCTTGTGGCAAGATGGCAAACTGGTGTAGAACCATGTC30.1654715940430226No Hit
GTTGGCGGATACCCTTCCAAGATGTGGCAAGATGGCAAACTGGTGTAGAA30.1654715940430226No Hit
GTTGGCGGATACCCTTCATTCAAGATGTGGCAAGATGGCAAACTGGTGTA30.1654715940430226No Hit
GTTGGCGGATACCCTTCCATTCAAGATGTGGCAAGATGGCAAACTGGTGT30.1654715940430226No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGAACAGATGTGGCAAGATGGCA30.1654715940430226No Hit
GTTGGCGGATACCCTCCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA30.1654715940430226No Hit
GTTGGCGGATACCCTTGTCTGTGTTGGCGGATACCCTTGTCTGTGTTGGC30.1654715940430226No Hit
TACTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG30.1654715940430226No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGGGCGAAGA30.1654715940430226Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGTGGCAAGATGGCAGATGTGGCAGATGGCAAACT20.11031439602868175No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGTCCTCAAC20.11031439602868175No Hit
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACCCTTGCTGTGTTGGCGG20.11031439602868175No Hit
GTTGGCGGATACCCTTCCATCTTAACAGAACAGATGTGGCAAGATGGCAA20.11031439602868175No Hit
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGA20.11031439602868175No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGGGCTAAGA20.11031439602868175Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATAAGATTCCTCAACTGGTGTAGAACCATGTCG20.11031439602868175No Hit
TACCTCAGTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAA20.11031439602868175No Hit
GTTGGCGGATACCCTTCCCCCTAAGATGTGGCAAGATGGCAAACTGGTGT20.11031439602868175No Hit
TACCTAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCGT20.11031439602868175No Hit
GTTGGCGGATACCCTTGCCTGTGTTGGCGGATACCCTGCTTCCCATGTAC20.11031439602868175No Hit
GTGGCGGATACCCTTCCCGCAGGCGGTTTGTTAAGTAAGATGTGCAAGAT20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGTT20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA20.11031439602868175No Hit
GTTGGCGGATACCCTTCCATTCCAGATGTGGCAAGATGGCAAACTGGTGT20.11031439602868175No Hit
GTTGGCGGATACCCTTCCACAGAGATGTGGCAAGATGGCAAACTGGTGTA20.11031439602868175No Hit
TACCTCAGTTTGCCCCCAGTGCACCATAAGATGTCCTCAACTGGTGTAGA20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTGCTGTGTTGGCGGAT20.11031439602868175No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCAAGATGTGGCAAGATG20.11031439602868175No Hit
GTTGCGGATACCCTTCCATAACACACGGGATAGCCTTCTGGCGCAGTCCA20.11031439602868175No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT20.11031439602868175Clontech SMART CDS Primer II A (96% over 26bp)
TACCTAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG20.11031439602868175No Hit
GTTGGCGGATACTAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCATG20.11031439602868175No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCAAGTC20.11031439602868175No Hit
GTTGGCGGATACCCTCCAGATGTGGCAAGATGGCAAACTGGTGTAGAACC20.11031439602868175No Hit
GTTGGCGGATACCCCAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCA20.11031439602868175No Hit
GTTGGCGGATACCCTCAGATGTGGCAAGATGGCAAACTGGTGTAGAACCA20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGGGGAT20.11031439602868175No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT20.11031439602868175No Hit
TACCTCAGTTTCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG20.11031439602868175No Hit
GTTGCGGATACCCTTGCTGGAAAGCAAGATGTGGCAAGATGGCAAACTGG20.11031439602868175No Hit
GTTGGCGGATACCCTTGTGTTGGCAGATGTGGCAAGATGGCAAACTGGTG20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTGTACTCTGCGTTGA20.11031439602868175No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACTAAGATGTGGCAAGA20.11031439602868175No Hit
TACCTCAGTTGCCCCCATGCAAACTCCGAATACCAGTAAGTACTATCCGG20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGGTGGCGGAT20.11031439602868175No Hit
GTTGGCGGATACCCTTGTGGCAAGATGGCAGATGTGGCAAGATGTCCTCA20.11031439602868175No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC20.11031439602868175Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGATACCCTTCCCGCAGGCGGTTTGTTAAGTCAGATGTGGCAAGA20.11031439602868175No Hit
TACCTCAGTTTGTCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC20.11031439602868175No Hit
GTTGGCGGATACCCTTCCATCTTACAGACAGATGTGGCAAGATGGCAAAC20.11031439602868175No Hit
GTTGGCGGATACCCTGTGTTGGCGGATACCCTGTGTTGGCGGATACCCTG20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCTGAT20.11031439602868175No Hit
TACCTCATTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG20.11031439602868175No Hit
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACTAAGATGTGGCAAGATG20.11031439602868175No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCCCATGTACTCTGCGTT20.11031439602868175No Hit
TACCTCAGTTTGCCCCCATGAGATGTGGCAAGATGGCAAACTGGTGTAGA20.11031439602868175No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCAGTGTTGGCGGAT20.11031439602868175No Hit
GTTGGCGGATACCTTTCATTCAAGATGTGGCAAGATGTCCTCAACTGGTG20.11031439602868175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)