FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19147
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1172261.221079020212045No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT226611.834752180498251No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG7043.676816211416932No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4072.125659372225414No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC2191.1437823157674831No Hit
TGCCATCTTGCCACATCTACAACAGGACCCAGCTGGCCAAGGGTATCCGC1070.5588342821329713No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC740.3864835222228025No Hit
TGCCATCTTGCCACATCTGATCTTGTTCTATCGGGTTTAAAGCAGTGGTA580.30291951741787226No Hit
GAGGACATCTTATGGTGCACTGGGGCCTTGTCTAGAATGCGCTCTGCATA530.27680576591633155No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC520.2715830156160234No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA490.255914764715099Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTATGGACTGCACATAGTCCTCAAAAGCAGTGGT460.24024651381417453No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA370.19324176111140126No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA340.17757351021047682No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT320.16712800960986054No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA310.1619052593095524No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT300.15668250900924427No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG290.15145975870893613No Hit
TGCCATCTTGCCACATCTTATCATCTCCCCATAAGACACTTGGTTGCATA280.14623700840862797No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT270.14101425810831983No Hit
TGCCATCTTGCCACATCTGAACCTCAGTTTCCTCATCTATAAGCAGTGGT270.14101425810831983No Hit
AAGGTGGATGGTGATGTGGTGGGGGAGGAGGAAGGCGGGTCCGGAAGGGG270.14101425810831983No Hit
TGCCATCTTGCCACATCTGTGTGAGCAGCTCAGAAAGCAGTGGTATCAAC270.14101425810831983No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA260.1357915078080117No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTACTTGGTTTCCCAGTCCACTA260.1357915078080117No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA250.13056875750770355No Hit
TGCCATCTTGCCACATCTTTCCACAAGGAGGAGTGAAACTCCAGACAAGT250.13056875750770355No Hit
TGCCATCTTGCCACATCTTACTCCCCAAGCAGTGGTATCAACGCAGAGTA250.13056875750770355Clontech Universal Primer Mix Long (100% over 24bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA240.12534600720739542No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG210.10967775630647099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACCA200.10445500600616284No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATCTGGTTTCCCAGTCCACTA200.10445500600616284No Hit
TGCCATCTTGCCACATCTGCTCCAAGCAGTGGTATCAACGCAGAGTACAT200.10445500600616284Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)