FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56831
Total Bases8.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2830549.8055638647921No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA991417.444704474670512No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT671611.81749397335961No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC30675.396702503915117No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA25214.435959247593743No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4300.7566293044289208No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3120.5489961464693566No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACCAGTCAA1560.2744980732346783No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC700.12317221234889408No Hit
TGCCATCTTGCCACATCTCTGCCTCCAGGCCAATAACTAGAGTCAGATCT660.11613380021467158No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT630.11085499111400468No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT620.10909538808044905No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA610.10733578504689342No Hit
TGCCATCTTGCCACATCTACACATAACCTCAGGAATCAGCCCTACACAAC590.10381657897978215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)