FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27531
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA777028.22273073989321No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT464916.886418945915512No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA400714.554502197522792No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC358413.01805237732011No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA23358.481348298281937No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC3341.2131778722167739No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2510.9116995387018271No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTA2390.868112309759907No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2180.791834659111547No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA1450.5266790163815336No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1000.3632269078493335No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT700.25425883549453343No Hit
TGCCATCTTGCCACATCTTTTACCAGAACGGCGATCAATCTTTTCCTTCA640.23246522102357342No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA570.2070393374741201No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT550.1997747993171334No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA540.1961425302386401No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACAGTCAA490.17798118484617342No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT470.17071664668918674No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC450.16345210853220007No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.14892303221822673No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC390.14165849406124004No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACCAGTCAA350.12712941774726672No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT330.11986487959028005No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC310.11260034143329338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)