FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27201
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1036038.08683504282931No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC747027.462225653468625No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT425415.639130914304623No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG6682.4557920664681445No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4271.5697952281166132No Hit
TGCCATCTTGCCACATCTGACCCCATGATATGTATTGTTAACTGACAGAA2911.0698136097937576No Hit
TGCCATCTTGCCACATCTTACCCCATGATATGTATTGTTAACTGACAGAA1100.40439689717289806No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT1040.3823388845998309No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC970.35660453659791924No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT640.23528546744604975No Hit
TGCCATCTTGCCACATCTTACTGAGGAAATAGTGTGGACGGCAGAGTAAC600.22058012573067165No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT570.2095511194441381No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC530.19484577772875997No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT460.16911142972684826No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA430.15808242344031467No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT360.132348075438403No Hit
TGCCATCTTCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT340.12499540458071395No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC320.11764273372302488No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC310.11396639829418037No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT300.11029006286533583No Hit
TGCCATCTTGCCACATCTTACATTTTCAAATAGGGTGAATCCTCTGTGTA280.10293739200764677No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)